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Purified anti-UFD1 Antibody

Pricing & Availability
Clone
W18140A (See other available formats)
Regulatory Status
RUO
Other Names
Ubiquitin Recognition Factor in ER-Associated Degradation 1, UB Fusion Protein 1, UFD1L, Ubiquitin Fusion Degradation Protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18140A_PURE_UFD1_Antibody_1_092419.png
Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-UFD1 antibody, clone W18140A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: molecular weight marker.
  • W18140A_PURE_UFD1_Antibody_1_092419.png
    Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-UFD1 antibody, clone W18140A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: molecular weight marker.
  • W18140A_PURE_UFD1_Antibody_2_092419.png
    Whole cell lysates (250 µg) were prepared from MOLT-4 cells and then immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) or Purified anti-UFD1 Antibody (clone W18140A). The resulting IP fractions and 5% whole cell lysate input were resolved by 4-12% Bis-Tris gel electrophoresis and probed with Purified anti-UFD1 Antibody (clone W18140A) followed by an HRP goat anti-rat IgG for detection.
  • W18140A_PURE_UFD1_Antibody_3_092419.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502, Panel A) or Purified anti-UFD1 Antibody, Clone W18140A (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
671301 25 µg £70
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671302 100 µg £174
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Description

UFD1 forms a ternary complex with Nuclear protein localization protein 4 homolog (NPLOC4) and valosin-containing Protein (VCP), and this complex plays an essential role in detecting and binding ubiquinated proteins. The complex also aids in the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear membrane after mitosis occurs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human anti-UFD1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1 µg per ml. For immunocytochemistry, a concentration of 1 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When stained for ICC, the methanol permeabilization method proved more effective than the Triton X-100 permeabilization method in localizing expression in the endoplasmic reticulum.

RRID
AB_2814530 (BioLegend Cat. No. 671301)
AB_2814530 (BioLegend Cat. No. 671302)

Antigen Details

Structure
UFD1 is a 307 amino-acid protein with a predicted molecular weight of 38 kD.
Distribution

Localized to endoplasmic reticulum; ubiquitously expressed

Function
Ubiquitinated protein degradation; mitotic spindle degradation; nuclear envelope formation
Interaction
NPLOC4; VCP
Cell Type
Dendritic cells
Biology Area
Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Antigen References
  1. Pizzuti A, et al. 1997. Hum. Mol. Genet. 6(2):259-65.
  2. Botta A, et al. 2001. Gene. 275(1):39-46.
  3. Hao Q, et al. 2015. EMBO. J. 34(23):2903-20.
  4. Chen M, et al. 2011. Proc. Natl. Acad. Sci. USA. 108(22):9119-24.
Gene ID
7353 View all products for this Gene ID
UniProt
View information about UFD1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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