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Purified anti-VEGFR2 Phospho (Tyr1175) Antibody

Pricing & Availability
Clone
Poly21A11 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor receptor 2, VEGF-receptor 2, Kinase insert domain receptor, KDR, CD309, Flk1
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly21A11_PURE_VEGFR2_Antibody_112222
Whole cell extracts (15 μg protein) from serum-starved HUVEC cells untreated (-) or treated (+) with 100 ng/mL Recombinant Human VEGF-165 (Cat. No. 583702) for 2 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with (1:2000 dilution) of purified anti-VEGFR2 (Tyr1175) (clone Poly21A11) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Equal VEGFR2 loading was confirmed by probing membranes with anti-pan VEGFR2 antibody. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly21A11_PURE_VEGFR2_Antibody_112222
    Whole cell extracts (15 μg protein) from serum-starved HUVEC cells untreated (-) or treated (+) with 100 ng/mL Recombinant Human VEGF-165 (Cat. No. 583702) for 2 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with (1:2000 dilution) of purified anti-VEGFR2 (Tyr1175) (clone Poly21A11) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Equal VEGFR2 loading was confirmed by probing membranes with anti-pan VEGFR2 antibody. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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610051 50 µL £201
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610052 200 µL £470
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Description

VEGFR2 is a tyrosine kinase receptor located in the cell surface. It serves as a receptor for VEGF-A, -E, -C, and -D. Interaction of VEGF and VEGFR2 induces dimerization of the receptor followed by transphosphorylation on its cytoplasmic domains. Phosphorylation sites include Tyr801, Tyr951, Tyr1054, Tyr1059, Tyr1175, Tyr1214, Tyr1223, Tyr1305, Tyr1309, and Tyr1319. Phosphorylation at the Tyr1175 residue facilitates binding for Phospholipase C-gamma (PLC-γ) and plays a critical role in signal transduction via MAP kinase pathway and DNA synthesis. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide corresponding to human VEGFR2 phosphorylated at Tyrosine 1175
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for Western Blot on lysates from Huvec cells stimulated with or without VEGF-165. 

RRID
AB_2936689 (BioLegend Cat. No. 610051)
AB_2936689 (BioLegend Cat. No. 610052)

Antigen Details

Structure
VEGFR2 is a 1,356 amino acid protein with a predicted molecular weight of 151 kD.
Distribution

Mostly endothelial cells. It is also upregulated in some cancer cells.

Function
Activation of VEGFR2 play essential role in angiogenesis, vascularization, cell proliferation, cell survival and migration. Its activation can be modulated by VEGFR1 that can sequester VEGF avoiding its binding to VEGFR2.
Interaction
VEGFR2 at Tyr1175 specifically interacts with phospholipase C-gamma leading activation of MAP kinase pathway.
Ligand/Receptor
VEGFR2 serves as a receptor for VEGF-A, -E, -C, and -D
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Phospho-Proteins
Antigen References
  1. Kanugula AK, et al. 2019. FASEB J. 33:195-203.
  2. Clegg LW, et al. 2015. PLoS Comput Biol. 11:e1004158.
  3. De la Paz NG, et al. 2013. Biochem Biophys Res Commun. 434:641-6.
Gene ID
3791 View all products for this Gene ID
UniProt
View information about VEGFR2 on UniProt.org
Go To Top Version: 1    Revision Date: 11/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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