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Purified anti-YY1 Antibody

Pricing & Availability
Clone
W21112E (See other available formats)
Regulatory Status
RUO
Other Names
Yin Yang 1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W21112E_PURE_YY1_WB_092223.
Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-YY1 (clone W21112E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W21112E_PURE_YY1_WB_092223.
    Whole cell extracts (15 µg total protein) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-YY1 (clone W21112E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W21112E_PURE_YY1_IHC-P_09052023
    IHC staining of purified anti-YY1 (clone W21112E) on formalin-fixed paraffin-embedded human colon. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. This was followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) at 2.5 µg/mL. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
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621551 25 µg £89
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621552 100 µg £264
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Description

Yin Yang 1 (YY1), is a ubiquitously distributed transcription factor that plays a pivotal role in regulating gene expression and coordinating various cellular processes. By interacting with other transcription factors, co-regulators, and epigenetic modifiers, YY1 can modulate gene expression to control diverse biological processes, including cell proliferation, differentiation, apoptosis, and development. Based on its interactions with other proteins such as MYC, YY1 can function as a transcriptional activator or repressor. Furthermore, YY1 is involved in maintaining genomic stability and has been implicated in DNA repair mechanisms. Its ability to interact with a wide range of regulatory elements and its context-dependent transcriptional activities make YY1 an essential factor in orchestrating intricate gene regulatory networks within the cell, and a potential target for cancer therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full-length human YY1 expressed in E.coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone is not recommended for ICC application.

For IHC-P antigen retrieval with either Tris-EDTA, pH 6.0, or sodium citrate, pH 9.0 (e.g. Cat. No. 420901) is recommended.

RRID
AB_3083409 (BioLegend Cat. No. 621551)
AB_3083409 (BioLegend Cat. No. 621552)

Antigen Details

Structure
YY1 is a 414 amino acid protein with a predicted molecular weight of 44.7 kD.
Distribution

Ubiquitous, Nuclear

Function
Transcription factor
Interaction
ATF6, EP300, FKBP3, HDAC3, HDAC2, Myc, NOTCH1, RYBP, SAP30, Yaf2
Biology Area
Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Weintraub AS, et al. 2017. Cell. 171:1573-1588.
  2. Kachigian LM. 2018. Int J Cancer. 143:460-465.
  3. Chen F. et al. 2017. Crit Rev Oncog. 22:131-141.
  4. Sarvagalla S, et al .2019. Front Oncol. 9:1230.
Gene ID
7528 View all products for this Gene ID
UniProt
View information about YY1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/05/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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