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Purified anti-ZAP-70 Antibody

Pricing & Availability
Clone
A16043B (See other available formats)
Regulatory Status
RUO
Other Names
Zeta chain of TCR associated protein kinase 70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16043B_PURE_ZAP-70_Antibody_052819.png
Human peripheral blood lymphocytes treated with True-Nuclear™ Transcription Factor Buffer Set, then stained with ZAP-70 (clone A16043B) Purified (filled histogram) or Purified Mouse IgG1, κ Isotype Ctrl (open histogram) followed by PE Goat anti-mouse IgG (minimal x-reactivity).
  • A16043B_PURE_ZAP-70_Antibody_052819.png
    Human peripheral blood lymphocytes treated with True-Nuclear™ Transcription Factor Buffer Set, then stained with ZAP-70 (clone A16043B) Purified (filled histogram) or Purified Mouse IgG1, κ Isotype Ctrl (open histogram) followed by PE Goat anti-mouse IgG (minimal x-reactivity).
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693502 100 µg £141
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Description

ZAP-70 is a 70kD protein expressed near the surface membrane of T-cells and NK cells. It is part of the intracellular signaling mechanism of the T-cell receptor, and plays integral role in T-cell signaling where it interacts with CD3-ζ to phosphorylate transmembrane protein LAT, which facilitates the binding of many signaling proteins. ZAP-70 plays a key role in the activation of T-cells, and their subsequent proliferation, differentiation, and production of cytokines in response to varied stimuli. It is used clinically as a prognostic marker helping differentiate the various types of chronic lymphocytic leukemia (CLL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP 70 peptide phosphorylated at Y493. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

CD3, CD19, and CD56 co-staining indicates that this clone does not bind B-cells and will not bind Syk. Clone A16043B cross-reacts with mouse species.

This clone, A16043B, recognizes both non-phosphorylated ZAP-70 and phosphorylated forms of ZAP-70.

RRID
AB_2810698 (BioLegend Cat. No. 693502)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains, 70 kD.
Distribution

T cells, NK cells, and B-cell chronic lymphocytic leukemia (B-CLL).

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Ligand/Receptor
Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc.
Cell Type
Leukemia, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
TCRs
Antigen References

1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC)
2. Crespo M, et al. 2003. N. Engl. J. Med. 348:1764. (WB, IP)
3. Jamroziak K, et al. 2009. Cancer Epidemiol. Biomarkers Prev. 18:945.

Gene ID
7535 View all products for this Gene ID 22637 View all products for this Gene ID
UniProt
View information about ZAP-70 on UniProt.org
Go To Top Version: 1    Revision Date: 05/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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