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Purified anti-ZAP70 Antibody

Pricing & Availability
Clone
A15114B (See other available formats)
Regulatory Status
RUO
Other Names
Zeta chain associated protein kinase, SYK related tyrosine kinase, ZAP-70
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_A15114B_PURE_ZAP70_Antibody_WB_090616
Total cell lysates (15 µg protein) from P116, Jurkat and EL-4 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-ZAP70 (clone A15114B) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 1_A15114B_PURE_ZAP70_Antibody_WB_090616
    Total cell lysates (15 µg protein) from P116, Jurkat and EL-4 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-ZAP70 (clone A15114B) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 2_A15114B_PURE_ZAP70_Antibody_WB_062218
    Total cell lysates (15 µg protein) from Ramos (lane 1), Jurkat (lane 2), EL4 (lane 3) and A20 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-ZAP70 antibody (clone A15114B) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for anti-ZAP70 antibody (upper blot) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower blot) antibody. Lane M: Molecular weight marker.
  • 3_A15114B_PURE_ZAP70_Antibody_ICFC_1_060617
    Human peripheral blood lymphocytes were surface stained with CD3 Brilliant Violet 421™, treated with True-Nuclear™ Transcription Factor Buffer Set, then intracellularly stained with purified anti-ZAP70 (clone A15114B) (top), or purified mouse IgG2b, κ isotype control (bottom), followed by mouse IgG2b, κ PE.
  • 4_A15114B_PURE_ZAP70_Antibody_ICFC_2_060617
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691202 100 µg £141
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Description

ZAP70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP70 have been linked to selective T cell defects. The ZAP70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-peptide surrounding ZAP70 Y319.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this antibody is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629829 (BioLegend Cat. No. 691202)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains. Human Zap70 has three isoforms with predicted MW of 70kD, 56kD, 36kD. Mouse Zap70 has three isoforms with predicted MW of 70kD, 36kD, 35kD.
Distribution

T cells, NK cells.

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Interaction
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated.
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1992. Cell. 71:649.
2. Chan AC, et al. 1994. Science. 264:1599.
3. Arpaia E, et al. 1994. Cell. 76:947.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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