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Purified anti-ZAP70 Phospho (Tyr292) Antibody

Pricing & Availability
Clone
A16038B (See other available formats)
Regulatory Status
RUO
Other Names
p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16038B_Purified_ZAP70_Phospho_Antibody_052919_updated.png
Human peripheral blood lymphocytes were treated with (left), or without (right), Hydrogen Peroxide for five minutes, fixed with Fixation Buffer permeabilized with True-Phos™ Perm Buffer, then surface stained with CD3 (OKT3) APC and intracellularly stained with purified anti-ZAP70 Phospho (Tyr292) (clone A16038B). Followed by anti-mouse IgG1, κ PE.
  • A16038B_Purified_ZAP70_Phospho_Antibody_052919_updated.png
    Human peripheral blood lymphocytes were treated with (left), or without (right), Hydrogen Peroxide for five minutes, fixed with Fixation Buffer permeabilized with True-Phos™ Perm Buffer, then surface stained with CD3 (OKT3) APC and intracellularly stained with purified anti-ZAP70 Phospho (Tyr292) (clone A16038B). Followed by anti-mouse IgG1, κ PE.
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693802 100 µg £197
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Description

ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.

ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr292 has a negative regulatory role.

Mutation of Tyr292 to phenylalanine enhanced NFAT induction in response to BCR stimulation, indicating phosphorylation of Tyr292 may promote association of ZAP-70 with a negative regulatory protein. ZAP-70 has been reported to bind Cbl following TCR stimulation and phosphorylation of Tyr292 was required for this interaction, as well as for the negative effect of Cbl on signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP70 peptide phosphorylated at Tyr292. Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Direct ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

During development, reactivity was observed with mouse phospho-SYK.

RRID
AB_2686945 (BioLegend Cat. No. 693802)

Antigen Details

Structure
619 amino acids with a predicted molecular weight of 70 kD.
Distribution

Cytoplasm; Human T Cells, Mouse T cells and B cells.

Function
Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.
Interaction
NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247/CD3Z.
Cell Type
B cells, T cells
Biology Area
Cell Biology, Immunology
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166.
2. Arpaia E, et al. 1994. Cell 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A, et al. 1993. Cell 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.
9. Magnan A, et al. 2001. J. Exp. Med. 194:491.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 Phospho Tyr292 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 04/25/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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