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Purified anti-ZAP70 Phospho (Tyr493) Antibody

Pricing & Availability
Clone
A16043C (See other available formats)
Regulatory Status
RUO
Other Names
SRK, STD, TZK, p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16043C_PURE_ZAP70Phospho_Antibody_091517
Whole cell extracts (15 µg total protein) prepared from Jurkat cells treated without (Lane 1) or with (lane 2) 2 mM H202 for 3 minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/ml (1:1000) purified anti-ZAP70 Phospho (Tyr493) antibody (Clone A16043C). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG. Equal ZAP70 loading was confirmed using purified anti-ZAP70 antibody used at 0.25 µg/ml (1:2000 dilution).
  • A16043C_PURE_ZAP70Phospho_Antibody_091517
    Whole cell extracts (15 µg total protein) prepared from Jurkat cells treated without (Lane 1) or with (lane 2) 2 mM H202 for 3 minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/ml (1:1000) purified anti-ZAP70 Phospho (Tyr493) antibody (Clone A16043C). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG. Equal ZAP70 loading was confirmed using purified anti-ZAP70 antibody used at 0.25 µg/ml (1:2000 dilution).
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620101 25 µg £77
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620102 100 µg £182
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Description

ZAP70 is a member of the Syk protein tyrosine kinase subfamily that is expressed exclusively in T cells and NK cells that plays an essential role in T cell receptor (TCR) signaling in combination with the Src family kinases, LCK and FYN. Upon TCR stimulation, phosphorylation of CD3 by LCK leads to the recruitment of ZAP70 to the activated receptor. Upon association of ZAP70 with the activated TCR complex, the kinase is phosphorylated on several tyrosine residues, including Tyrosine 493. Phosphorylation of Tyrosine 493, which is found within the activation loop of the protein, results in increased enzymatic activity. ZAP70 subsequently phosphorylates downstream targets, including the adaptor molecules LAT and LCP2, facilitating the recruitment and activation of other signaling molecules, ultimately leading to T cell proliferation, differentiation, and activation. ZAP70 mays also serve as a scaffold for recruiting additional factors to the activated TCR, and can function to regulate TCR-CD247/CD3Z expression at the plasma membrane. ZAP70 deficiency results in severely impaired immune function, while higher levels of ZAP70 expression are associated with certain B cell malignancies. PTN22 is required for the dephosphorylation of ZAP70 Tyrosine 493.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP70 peptide phosphorylated at Tyr493
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 ug/mL (1:1000-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2716044 (BioLegend Cat. No. 620101)
AB_2716044 (BioLegend Cat. No. 620102)

Antigen Details

Structure
619 amino acid protein with a predicted molecular weight of 70 kD. Contains 2 N-terminal SH2 domains and a C-terminal kinase domain.
Distribution

T cells and NK cells

Function
Protein tyrosine kinase, T cell signaling
Interaction
CD247/CD3Z, NFAM1, SLA, , FCRL3, VAV1, CBL, RHOH, DEF6
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Katz ZB, et al. 2017. Nat Immunol.18:86.
  2. Klammt C, et al. 2015. Nat Immunol. 16:961.
  3. Jenkins MR, et al. 2014. eLife. 3:e01310
  4. Boldizsar F, et al. 2013. Mol Immunol. 53:111.
  5. Wu J, et al. 2006.  J. Biol Chem. 281:11002.
  6. Kong G, et al. 1996. Mol. Cell. Biol. 16:5026.
  7. Wange RL, et al. 1995. J. Biol. Chem. 270:18730.
  8. Chan AC, et al. 1995. EMBO. J.14:2499.
Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 Phospho Tyr493 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 09/20/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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