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Purified F(ab')2 Goat anti-mouse IgM (μ chain) Antibody

Pricing & Availability
Clone
Poly21571 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
Poly21571_PUREAF_IgM_Antibody_1_090319
C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_1_090319
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_2_082919
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD86 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_3_082919
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 2 minutes, fixed with Fixation buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, then stained with B220 Brilliant Violet 421™ and ZAP70 Phospho (Tyr319)/Syk (Tyr352) PE
Cat # Size Price Quantity Check Availability Save
157102 500 µg £189
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Description

Poly21571 reacts with mouse IgM. It can be used in functional assays, such as B cell activation. It has minimum cross-reactivity with bovine proteins. The optimal working concentration should be determined empirically based on the specific assay of interest.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
Preparation
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. The solution contains no preservative; handle under aseptic conditions.
Application

Activ - Quality tested
Recommended Usage

This product was QC tested by stimulating C57BL/6 splenic B cells. The recommended working concentration is 10 - 20 µg/mL

Product Citations
  1. Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
  2. Wu Q, et al. 2023. J Virol. e0065623:97. PubMed
RRID
AB_2814087 (BioLegend Cat. No. 157102)

Antigen Details

Cell Type
B cells
Biology Area
Immunology, Signal Transduction
Molecular Family
Adaptor Proteins
Gene ID
16019 View all products for this Gene ID
Go To Top Version: 1    Revision Date: 08/29/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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