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Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm)

Pricing & Availability
Regulatory Status
RUO
Other Names
Sphero™ brand beads, 8 peak Rainbow Calibration Beads
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Product Citations
publications
RCP_8-peak_1_080812
Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).
  • RCP_8-peak_1_080812
    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).
  • RCP_8-peak_2_080812
    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel) plotted against bead fluorescence at 405 nm excitation with a 450/50 BP filter (BV421™ channel).
  • RCP_8-peak_3_080812
    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel) plotted against bead fluorescence at 405 nm excitation with a 450/50 BP filter (BV421™ channel).
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422903 5 mL £201
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Description

These Rainbow Calibration Particles are a mixture of 3.0 - 3.4 μm particles, dyed to eight different fluorescent intensities. As their excitation range is 365 - 650 nm, they can be used with most lasers. However, they are not for use with the UV laser. The emission spectra of these rainbow particles is compatible with many common fluorophores used in immunofluorescent staining with flow cytometric analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Rainbow Calibration Particles are in deionized water with 0.02% sodium azide and 0.01% NP-40.
Concentration
107 particles per ml.
Storage & Handling
Store between 2°C and 8°C. Do not freeze. Protect from light.
Application

FC
Recommended Usage

Resuspend well (e.g. vortex briefly) before adding 3-5 drops to 1 ml of filtered DI water.

Application Notes

Use of Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm), is common for the following purposes:
1. Instrument performance verification (linearity) and monitoring
2. Standardization of instrument settings for longitudinal studies (can also use Rainbow Calibration Particles, 6 peaks (Cat. No. 422901), and single-peak Rainbow Fluorescent Particles (Cat No. 422905 and 422907)
3. Evaluation of new collection optics (e.g. bandpass filters)



The relative number of fluorophores per particles has been determined for every peak of this product in FL1 (FITC and MEFL), FL2 (RPE and MEPE), FL3 (RPECy5 and MEPCY) and FL4 (APC and MEAP) channels on a flow cytometer to plot the calibration graph, which can be used to check linearity of the PMT in each channel. PMT QC templates can be downloaded directly from Spherotech™.

Application References

(PubMed link indicates BioLegend citation)
  1. Calibration and Performance Tracking of Flow Cytometers Using Sphero™ Calibration Particles
  2. Measuring Molecules of Equivalent Fluorochrome (MEF) Using Sphero™ Rainbow and Ultra Rainbow Calibration Particles
  3. Determining PMT Linearity in Flow Cytometers Using the Sphero™ PMT Quality Control Excel Template
  4. Perfetto SP, et al. 2006. Nat. Protoc. 1:1522.
Product Citations
  1. Fedorec AJH, et al. 2020. ACS Synth Biol. 2258:9. PubMed
  2. Bernal C, et al. 2020. Cell Rep. 108372:33. PubMed
  3. Clottu A, et al. 2017. Cell Rep. 18(1):213-224. PubMed
  4. Sørensen MES, et al. 2020. Curr Biol. 328:30. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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