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- Regulatory Status
- RUO
- Other Names
- Bone Morphogenetic Protein 6, BMP6, VGR1, VGR, VGR-1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human BMP-6 induces the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL. -
Stability Testing for Recombinant Human BMP-6. Recombinant human BMP-6 was aliquoted in 0.1% Trifluoroacetic acid (TFA) at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to induce the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 569304 | 25 µg | £394 | ||||
| 569306 | 100 µg | £749 | ||||
Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four Type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human BMP-6 can restore bone in animal models of osteoporosis and induce bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. Human BMP-6 has been reported to have an antiproliferative effect on human B, human CD4 T, Jurkat cells and mouse peritoneal macrophages. In addition, BMP-6 induces expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).
Product DetailsProduct Details
- Source
- Recombinant Human BMP-6, amino acid sequence (Gln382-His513) (Accession: # P22004), was expressed in CHO cells. The amino-terminus contains 6His-GGS tag.
- Molecular Mass
- The 132 amino acid recombinant protein has a predicted molecular mass of approximately 14.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 18 kD and 40 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 0.1% Trifluoroacetic acid (TFA)
- Endotoxin Level
- Less than 0.1 EU per µg of cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human BMP-6 induces the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Oocytes, granulosa cells, bone marrow-mesenchymal stem cells, hematopoietic stem cells, embryonic stem cells, mesenchymal stem cells, neural stem cells, corneal epithelial, and osteoclasts
- Function
- Osteoblast differentiation, bone regeneration. Controls the self-renewal and differentiation of stem cells. Antiproliferative effect on B, T cells, and macrophages. Regulates hepcidin expression and iron metabolism in vivo.
- Interaction
- Cancer cells, mesenchymal stem cells, germline stem cells, hematopoietic stem cells, and neural stem cells
- Ligand/Receptor
- BMP receptors Alk-2 and Alk-3 (type I), BMPRII (type II)
- Bioactivity
- BMP-6 induces alkaline phosphatase in ATDC-5 mouse chondrogenic cell line.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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- Chen D, et al. 2004. Growth Factors. 22:233-41.
- Kertein C, et al. 2005. BMC Immunol. 6:9.
- Grasser WA, et al. 2007. Int Orthop. 31:759-65.
- Sivertsen EA, et al. 2007. Eur J Immunol. 37:2937-48.
- Andriopoulos B, et al. 2009. Nat Genetics. 41:482-7.
- Hong JH, et al. 2009. Immunology. 128:e442-50.
- Lee GT, et al. 2011. Mol Immunol. 48:1540-7.
- Shen R, et al. 2015. Curr Pharm Des. 21:3634-43.
- Tiwari A, et al. 2020. Invest Ophthalmol Vis Sci. 61:46.
- Gene ID
- 654 View all products for this Gene ID
- UniProt
- View information about BMP-6 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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