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- Regulatory Status
- RUO
- Other Names
- CD22 Antigen, SIGLEC2, SIGLEC-2, BL-CAM, B-Cell Receptor CD22
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- Product Citations
- publications
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Recombinant Human CD22-Fc chimera supports the adhesion of human red blood cells (RBC). The ED50 for this effect is 0.9 –4.5 µg/mL. -
Stability Testing for Recombinant Human CD22-Fc Chimera. Recombinant human CD22-Fc chimera was aliquoted in PBS, 0.5 M NaCl, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their capacity to support the adhesion of human RBC. The ED50 for this effect is 0.9 –4.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 562554 | 25 µg | £148 | ||||
| 562556 | 100 µg | £353 | ||||
CD22 is a type I transmembrane protein of 140 kD expressed in B cells. The extracellular domain (ECD) contains seven Ig domains with 12 recognized N-linked glycosylation sites. CD22 binds to α2,6-linked sialic acid (Sias) ligands connected to galactose. The Ig domain 1 (d1) recognizes Sias containing α2,6-linkages. CD22 regulates signaling through its intracellular inhibitory ITIMs domains. The regulation of B cell function by CD22 is performed through sialic ligand-dependent and -independent mechanisms. CD22 regulates the migration of recirculating B cells to the bone marrow. CD22 knockdown mice present B cell impaired survival, increase of B cell turnover, and decrease of marginal zone B cells. In addition, CD22-deficient B cells show hyperactivation in response to TLR (TLR3, TLR4, and TLT9) ligands. CD22 ligation with anti-CD22 affects the response to TLR7. Under this condition, the B cell differentiation into plasmablasts is limited, and the B cell production of cytokine by the stimulation of TLR7 or TLR9 is affected. B-cell leukemias and lymphomas express CD22, and it has been used as a target for Ab-dependent therapies. Epratuzumab (Emab) is a humanized anti-CD22 antibody used in clinical trials of B-cell Non-Hodgkin's lymphoma (NHL) and acute lymphoblastic leukemia (ALL). In addition, Emab has been explored in clinical trials for SLE and primary Sjogren’s syndrome.
Product DetailsProduct Details
- Source
- Human CD22, amino acid sequence (Asp20-Arg687) (Accession: # P20273), was expressed in CHO cells. The carboxy-terminus contains IEGRMD-hIgG1-(Pro100-Lys330)-Fc tag.
- Molecular Mass
- The 905 amino acid recombinant protein has a predicted molecular mass of approximately 101.8 kD. The DTT-reduced and non-reduced proteins migrate at approximately 115 - 140 kD and 140 - 260 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, 0.5M NaCl, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg of cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human CD22-Fc chimera supports the adhesion of human red blood cells (RBC) in a dose-dependent manner. The ED50 for this effect is 0.9 – 4.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
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B cells
- Function
- Mediates B cell/B cell interactions, regulates B cell functions, inhibitory co-receptor of BCR, regulate negatively TLRs signaling in B cells
- Interaction
- B cells, B cell lines, DC, RBC
- Ligand/Receptor
- CD45RO, CD75
- Bioactivity
- Human CD22-Fc supports the adhesion of human RBC.
- Cell Type
- B cells
- Biology Area
- Adaptive Immunity, Cell Adhesion, Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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- Willson GL, et al. 1991. J Exp Med. 173:137-46.
- Santos L, et al. 2008. J Immunol. 180:4561-9.
- Nirihito K, et al. 2011. J Innate Immune. 3:411.
- Poe JC and Tedder TF. 2012. Trends Immunol. 33:413-20.
- Muller J, et al. 2013. Proc Natl Acad Sci USA. 110:12402-7.
- Oreno-Orbea J, et al. 2017. Nat Commun. 8:764.
- Giltiay NV, et al. 2017. Arthritis Res Ther. 19:91.
- Clark EA and Giltiay NV. 2018. Front Immunol. 9:2235.
- Gottenberg JE, et al. 2018. Arthr Rheumatol. 70:763-773.
- Gene ID
- 933 View all products for this Gene ID
- UniProt
- View information about CD22 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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