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- Regulatory Status
- RUO
- Other Names
- CLEC2D, C-type lectin domain family 2 member D, C-type lectindomain family 2, member D, C-type lectin related f, Lectin-like NKcell receptor, Lectin-like transcript 1, LLT1, LLT1 Osteoclastinhibitory lectin, OCIL, OCILmember D, CLAXLLT-1
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- Product Citations
- publications
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When recombinant human CLEC2D/OCIL is immobilized at 2 µg/mL, biotinylated human CD161/CLEC5B binds in a dose-dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 range for this effect is 0.1 – 0.7 µg/mL. -
Stability testing for Recombinant Human CLEC2D/OCIL. Recombinant Human CLEC2D/OCIL was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized recombinant human CLEC2D/OCIL at 2 µg/mL and different concentration of biotinylated human CD161/CLEC5B that binds in a dose-dependent manner. The EC50 range for this effect is 0.1 – 0.7 µg/mL .
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 565104 | 25 µg | £236 | ||||
| 565106 | 100 µg | £483 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
C-type lectin-like domain family 2-member D (CLEC2D), also known as osteoclast inhibitory lectin (OCIL) and lectin-like transcript 1 (LLT1), is a member of the natural killer cell receptor C-type lectin family. It plays an important role in inflammation and immunotherapy. CLEC2D is primarily expressed by B cells and monocytes in peripheral blood, antigen presenting cells, activated lymphocytes, chondrocytes, and osteoblasts. CLEC2D binds sulfated glycosaminoglycans, inhibits bone degradation, osteoclast formation, and the ability of NK cells to target glioma and prostate cancer cells. Overexpression of CLEC2D on prostate cancer cells inhibits NK cell-mediated killing by interacting with Killer cell lectin like receptor B1/cluster of differentiation 161 (CD161).
Product DetailsProduct Details
- Source
- Recombinant human CLEC2D/OCIL, amino acid Arg60 - Val191 (Accession No. Q9UHP7) was expressed in 293E cells.
- Molecular Mass
- The 376 amino acid recombinant protein has a predicted molecular mass of approximately 42.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 50 – 62 and 80 – 115 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Arg.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant human CLEC2D/OCIL at 2 µg/mL binds to biotinylated recombinant CD161/CLEC5B in a dose-dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.7 µg/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Hematopoietic cell lines, activated lymphocytes (NK cells, T cells, B cells), and antigen presenting cells
- Function
- Receptor for Killer cell lectin like receptor B1, inhibits osteoclast formation and bone resorption
- Interaction
- NK cells, T cell, Thymocytes
- Ligand/Receptor
- CD161(CLEC5B)
- Bioactivity
- Measured by its ability to bind CD161/CLEC5B
- Cell Type
- B cells, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, NK cells, T cells
- Biology Area
- Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- Innate Immune Signaling
- Antigen References
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- Germain C, et al. 2010. J Biol Chem. 285:36207-15.
- Rosen DB, et al. 2005. J Immunol. 175:7796-9.
- Gange CT, et al. 2004. J Biol Chem. 279:29043-9.
- Hu YS, et al. 2004. J Bone Miner Res. 19:89-99.
- Lai J, et al. 2020. Immunity. 52:123.
- Sun Y, et al. 2019. J Cancer Metastasis Treat. 5:80.
- Gene ID
- 29121 View all products for this Gene ID
- UniProt
- View information about CLEC2D/OCIL on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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