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- Regulatory Status
- RUO
- Other Names
- CLEC6A, dectin 2, Dectin2, Dectin-2, CLEC4N, CLECSF10, C-typelectin domain family 6 member A, C-type lectin superfamilymember 10, DC-associated C-type lectin 2, Dendritic cellassociated C-type lectin 2
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- Product Citations
- publications
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When recombinant human CLEC6A/Dectin-2 is immobilized at 2 µg/mL, human FcγRI binds in a dose-dependent manner. HRP anti-His Tag (Cat. No. 652504) was used to detect the binding. The ED50 for this effect is 3 – 21 ng/mL. -
Stability testing for Recombinant Human CLEC6A/Dectin-2-Fc. Recombinant Human CLEC6A/Dectin-2-Fc was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized recombinant human CLEC6A/Dectin-2 at 2 µg/mL and different concentration of human FcγRI that binds in a dose-dependent manner. The EC50 for this effect is 3 – 21 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 565004 | 25 µg | £202 | ||||
| 565006 | 100 µg | £425 | ||||
C-type lectin domain family 6, member A (CLEC6A), also known as Dectin-2, is a glycosylated type II transmembrane protein. It is expressed on monocytes, macrophages, dendritic cells (DCs) and CD4+ T cells. It plays a key role in host defense against fungal infections via the Th17 response. It binds high-mannose structures in a wide range of species including yeast and fungi. It also interacts with the Immunoreceptor tyrosine-based activation motif (ITAM)-containing adaptor protein Fc receptor gamma subunit on macrophages. The ligation of CLEC6A induces tyrosine phosphorylation of Fc receptor gamma chain and activation of NF kappa B. Activated CLEC6A can induce production of cytokines, including IL-6, IL-23, and IL-1β.
Product DetailsProduct Details
- Source
- Recombinant Human CLEC6A/Dectin-2, amino acid Thr46-Leu209 (Accession No. Q6EIG7) was expressed in CHO cells.
- Molecular Mass
- The 403 amino acid recombinant protein has a predicted molecular mass of approximately 45.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 55 and 100 kD by SDS-PAGE, respectively.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant human CLEC6A/Dectin-2 at 2 µg/mL binds to recombinant FcγRI/CD64 in a dose-dependent manner. HRP anti-His Tag (Cat. No. 652504) was used to detect the binding. The ED50 for this effect is 3 – 21 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Type II transmembrane C-type lectin, one carbohydrate recognition domain, ITAM containing cytoplasmic tail
- Distribution
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Macrophages, dendritic cells, peripheral blood monocytes, neutrophils
- Function
- Mediates innate immunity to fungal pathogens
- Interaction
- C. albicans, C. glabrata, fungi, bacteria
- Ligand/Receptor
- FcγRI, α-mannans
- Bioactivity
- Measured by its ability to bind FCγRI
- Cell Type
- Dendritic cells, Macrophages, Neutrophils
- Biology Area
- Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- Innate Immune Signaling
- Antigen References
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- Taylor PR, et al. 2014. Nature Immunol. 15:143-51.
- Onyishi CU, et al. 2022. Immunology. 165: 143-157.
- Borriello F, et al. 2020. Eur J Immunol. 50: 317-325.
- Sato K, et al. 2006. J Biol Chem. 281: 38854-66.
- Carter RW, et al. 2006. Cell Immunol. 239:87 -91.
- Yonekawa A, et al. 2014. Immunity. 41:402-413.
- Graham LM, et al. 2009. Cytokine. 48: 148-55.
- Gene ID
- 93978 View all products for this Gene ID
- UniProt
- View information about CLEC6A/Dectin-2 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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