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- Regulatory Status
- RUO
- Other Names
- Frizzled Class Receptor 8, Frizzled 8, Seven Transmembrane Spanning Receptor, Frizzled Family Receptor 8, Frizzled Homolog 8 (Drosophila), Frizzled Homolog 8, FZ-8, HFZ8, Fz-8, HFz8
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human Frizzled-8-Fc chimera inhibits Topflash reporter activity induced by 50 ng/mL recombinant mouse Wnt3a (Cat. No. 772301) in a dose-dependent manner. ED50 for this effect is 80 – 420 ng/mL. -
Stability Testing for Recombinant Human Frizzled-8-Fc Chimera. Recombinant human Frizzled-8-Fc chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested for their ability to inhibit Topflash reporter activity induced by 50 ng/mL recombinant mouse Wnt3a (Cat. No. 772301).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 795504 | 25 µg | £133 | ||||
| 795506 | 100 µg | £462 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Frizzled-8 (FZD8), a G-protein coupled receptor with seven transmembrane domains (7TM), is one member of ten Frizzled protein family. Frizzled proteins are the principal receptors for the Wnt family of ligands, mediating canonical Wnt signaling. Frizzled proteins are present in most of the animals, but not present in plants or in simpler (single cell) eukaryotes. In the presence of Wnt ligand, Wnt binds to extracellular cysteine rich domain (CRD) of Frizzled. A receptor complex forms between Frizzled and low-density lipoprotein receptor-related protein 5/6 (LRP5/6). Subsequently, Dishevelled (Dvl) recruitment by Frizzled leads to LRP5/6 phosphorylation and recruitment of scaffolding protein, Axin. This disrupts Axin-mediated phosphorylation/degradation of β-catenin, allowing β-catenin to accumulate in the nucleus where it serves as a coactivator for TCF to activate Wnt-responsive genes. Human FZD-8 contains 694 amino acids (aa) with a 248 aa extracellular domain (ECD) (aa 28-275) that includes a highly conserved cysteine-rich region (CRD), the 7TM region (aa 276-605), and a C-terminal cytoplasmic domain (aa 606-694). According the identity of aa sequences, frizzled proteins can be divided into four subgroups: subfamily 1 (FZD1, FZD2, and FZD7), subfamily 2 (FZD5 and FZD8), subfamily 3 (FZD9 and FZD10), subfamily 4 (FZD4) and subfamily 5 (FZD3 and FZD 6). FZD8 shares about 70% identity with other frizzled proteins. hFZD-8 is abundant expressed in fetal kidney and also expressed in fetal brain, fetal lung and adult kidney, adult heart, adult pancreas and adult skeletal muscle. Knockout of FZD8 in mice has no apparent phenotype, but leads to reduced ureteric bud growth and a reduction in kidney size. It has been reported that FZD8 can promote the cell proliferation and metastasis of renal cell carcinoma. Targeting Human FZD-8 suppresses the migration and invasion of breast cancer and non-small cell lung cancer (NSCLC) cells through inhibiting Wnt/β-catenin signaling pathway. FZD-8 also participates in non β-catenin signaling pathway. FZD8 integrates Wnt-11 and TGF-β signals to promote epithelial-to-mesenchymal transition (EMT) independently of β-catenin. FZD-8 can serve as a potential therapeutic target in many human cancers.
Product DetailsProduct Details
- Source
- Human Frizzled-8, amino acid Ala25-Pro172 (Accession: #Q9H461), with a C-terminal human IgG1 (Pro100-Lys330) Fc, was expressed in CHO cells.
- Molecular Mass
- The 387 amino acid recombinant protein has a predicted molecular mass of approximately 43.61 kD. The DTT-reduced and non-reduced protein migrates at approximately 56 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 80 – 420 ng/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by 50 ng/mL of recombinant mouse Wnt3a (Cat. No. 772301).
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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Expressed in fetal kidney, brain, lung, kidney, heart, pancreas and skeletal muscle of adult
- Function
- Receptor for canonical Wnt signaling pathway, angiogenesis, cell differentiation
- Ligand/Receptor
- Wnt proteins, LRP5, LRP6, Glypican-3
- Bioactivity
- Measured by its ability to inhibit Wnt3a activity in Topflash assay
- Cell Type
- Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Stem Cells
- Molecular Family
- Soluble Receptors
- Antigen References
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- Goodfellow PN, et al. 1988. Philos Trans R Soc Lond B Biol Sci. 322:145-54.
- Husak Z, et al. 2012. Cell Death Dis. 3:e425.
- Lou O, et al. 2007. J Immunol. 178:1136-43.
- Manara MC, et al. 2006. Mol Biol Cell. 17:1910-21.
- Park SH, et al. 2005. Gene. 353:177-88.
- Pasello M, et al. 2018. J Cell Commun Signal. 12:55-68.
- Rocchi A, et al. 2010. J Clin Invest. 120:668-80.
- Schenkel AR, et al. 2002. Nat Immunol. 3:143-150.
- Scotlandi K, et al. 2006. Eur J Cancer. 42:91-6.
- Watson R.L, et al. 2015. J Exp Med. 212:1021-41.
- Gene ID
- 8325 View all products for this Gene ID
- UniProt
- View information about Frizzled-8 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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