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Recombinant Human IGFALS (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
ACLSD, ALS, insulin-like growth factor binding protein, acid labile subunit
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RECOM_HU_IGFALS_ELISA_STD_122618
  • RECOM_HU_IGFALS_ELISA_STD_122618
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758809 4 pack £65
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Description

The insulin-like growth factors (IGFs) are found in binary complexes with members of IGF-binding protein family (IGFBP-1 to -6). IGFALS is the acid-labile subunit of the IGF binding protein complex that forms ternary complexes with IGFs and IGFBP-3/IGFBP-5. IGFALS has no affinity for free IGFs and has a very low affinity for uncomplexed IGFBP-3/IGFBP-5, but readily binds to the IGFs and IGFBP-3/IGFBP-5 complex, which extends the serum half-lives of IGFs. IGFALS is mainly produced by the liver and is exclusively found in high concentration in postnatal serum. IGFALS production is predominantly stimulated by the growth hormone. Mutations in IGFALS causes ALS, amyotrophic lateral sclerosis, deficiency, which manifests itself as delayed and slow puberty. IGFALS mutations have also been associated with insulin resistance. Mature IGFALS protein is composed of 578 amino acids with 20 leucine-rich repeats. It shares 93% identity between mouse and rat, and 79% between mouse and human.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human IGFALS, amino acids (Ala28 -Cys605) (Accession# NP_002382.1), was expressed in CHO cells.
Molecular Mass
The 593 amino acid recombinant protein has a predicted molecular mass of approximately 65 kD. The protein migrates at approximately 75 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 10% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA - Quality tested
Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 3.75 - 120 ng/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IGFALS protein is useful as a standard for a human IGFALS sandwich ELISA, using unlabeled M6005C04 antibody (Cat. No. 537302) as capture and biotinylated M6001E07 antibody (Cat. No. 537404) as detection.

Antigen Details

Distribution

Produced by the liver and is mainly found in the postnatal serum.

Function
IGFALS production is stimulated by growth hormone and is important for extending the serum half life of IGF.
Interaction
IGF-1/IGF-2 and IGFBP-3/IGFBP-5 binary complexes.
Ligand/Receptor
IGF-1/IGF-2 and IGFBP-3/IGFBP-5 binary complexes.
Bioactivity
Forms ternary complex with IGFs and IGFBP-3/IGFBP-5 binary complexes.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Carrier Proteins
Antigen References
  1. Baxter RC, Dai J. 1994. Endocrinology. 134:848-852.
  2. Twigg SM, Baxter RC. 1998. J Biol Chem. 273(11):6074-9.
  3. Khosravi MJ, et al. 1997. J Clin Endocrinol Metab. 82:3944-3951.
  4. Domene HM, et al. 2004. New Eng J Med. 350:570-577.
  5. Domene HM, et al. 2005. J Endocrinol Invest. 28(5):43-6.
Gene ID
3483 View all products for this Gene ID
UniProt
View information about IGFALS on UniProt.org
Go To Top Version: 1    Revision Date: 12/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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