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- Regulatory Status
- RUO
- Other Names
- Mucin 1, EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227
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- Product Citations
- publications
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When recombinant human MUC-1 (CD227) is immobilized at 2 µg/mL, it binds recombinant human Siglec-9-Fc chimera in a dose-dependent manner. The ED50 for this effect is 1.6 – 8 µg/mL -
Recombinant human MUC-1 (CD227) was aliquoted in PBS, pH 7 at 0.2 mg/mL and one aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind human Siglec-9-Fc in a dose- dependent manner, when recombinant human MUC-1 is immobilized at 2 µg/mL. The ED50 for this effect is 1.6 – 8 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 797304 | 25 µg | £153 | ||||
| 797306 | 100 µg | £478 | ||||
MUC-1 (CD227) is a member of the mucin family of proteins, characterized by high carbohydrate content (O-glycosylation) and been part of protective mucous barriers on epithelial surfaces. MUC-1 is bound to the membrane on the apical surface of epithelial cells and consists of a heterodimer formed by alpha (N-terminal) and beta (C-terminal) subunits, which are produced from proteolytic cleavage. The alpha subunit carries cell adhesion properties, while the beta subunit is involved in cell signaling. The MUC-1 gene contains a highly polymorphic variable number repeat domains, and alternative splicing yields multiple transcript variants. One variant, known as Y-LSP, lacks the mucin repeats and has been shown to inhibit tumor growth in mice. MUC-1 aberrant expression, cellular localization, and glycosylation have been associated with carcinomas. High level of MUC-1 expression has been linked with metastasis and poor prognosis. MUC-1, also known as Krebs von den Lungen-6 (KL-6), has been detected at high levels in serum of patients with interstitial lung diseases such as allergic pneumonia and idiopathic pulmonary fibrosis. MUC-1 has been used as a biomarker of acute respiratory distress syndrome (ARDS). Critically ill patients with COVID-19 often progress to ARDS, and MUC-1 has been connected with mortality and severity in patients with COVID-19.
Product DetailsProduct Details
- Source
- Human MUC-1 (CD227), amino acid (Pro24-Gly167) (Accession: P15941), with a C-terminal His-TG-8His-GGQ, was expressed in 293E cells.
- Molecular Mass
- The 157 amino acid recombinant protein has a predicted molecular mass of approximately 16.9 kD. The protein migrates at approximately 27 – 30 kD by SDS-PAGE in DTT-reduced and non-reduced conditions. The predicted N-terminal amino acid is Pro.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human MUC-1 (CD227) is immobilized at 2 µg/mL, it binds recombinant human Siglec-9-Fc chimera in a dose-dependent manner. The ED50 for this effect is 1.6 – 8 µg/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Heterodimer (alpha and beta subunit)
- Distribution
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Epithelial cells, alveolar type II cells, adenocarcinomas, human myeloma cells, activated human T cells
- Function
- Immuno-regulatory role in infections, inflammation, and autoimmunity. Cell-cell and cell-extracellular matrix interactions.
- Interaction
- GRB2 (isoform 1), ICAM-1 (core), SRC (cytoplasmic tail), DC-SING (CD209), macrophage galactose lectin (MGL), siglec-1 (CD169), galectin-3, and E-selectin
- Ligand/Receptor
- Siglec-9
- Bioactivity
- Human MUC-1 (CD227) binds to recombinant human Siglec-9-Fc chimera in a dose-dependent manner.
- Cell Type
- Dendritic cells, Epithelial cells, Lymphocytes, Macrophages, Monocytes, T cells
- Biology Area
- Angiogenesis, Cancer Biomarkers, Cell Adhesion, Cell Biology, Immuno-Oncology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Ligtenberg MJ, et al. 1992. J Biol Chem. 267:6171-7.
- Bafna S, et al. 2010. Oncogene. 29:2893-904.
- Zhang L, et al. 2013. Cancer Immunol ImmunoTher. 62:423-35.
- Agrawal A, et al. 2018. Front Immunol. 9:2391.
- Yamaya T, et al. 2021. Respir Investig. 59:596-601.
- Chen H, et al. 2021. Cytokine. 148:155513.
- Gene ID
- 4582 View all products for this Gene ID
- UniProt
- View information about MUC-1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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