Recombinant Human PD-L1 (carrier free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
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PD-L1-_Human_RECOM_BA_1_041523
Biotinylated Recombinant Human PD-1 (CD279)-Fc (hPD-1) (Cat. No. 789402) binds to immobilized recombinant human PD-L1 in a dose-dependent manner. Recombinant human PD-L1 was immobilized using purified anti-His Tag antibody (Cat. No. 652501). HRP-Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.6 µg/mL.
  • PD-L1-_Human_RECOM_BA_1_041523
    Biotinylated Recombinant Human PD-1 (CD279)-Fc (hPD-1) (Cat. No. 789402) binds to immobilized recombinant human PD-L1 in a dose-dependent manner. Recombinant human PD-L1 was immobilized using purified anti-His Tag antibody (Cat. No. 652501). HRP-Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.6 µg/mL.
  • PD-L1-_Human_RECOM_BA_2_041523.
    Stability testing for recombinant human PD-L1. Recombinant human PD-L1 was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to bind to biotinylated recombinant human PD-1 in a dose dependent manner. HRP-Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.6 µg/mL.
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560104 25 µg £124
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560106 100 µg £219
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

PD-L1, a member of the B7 family, is a type I transmembrane protein of 290 amino acids. Human PD-L1 has 70% amino acid identity to its mouse orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PD-L1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model. Antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors, and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore the antitumor immune response. Antibodies targeting PD-1 or PD-L1 block the PD-1 pathway and reactivate T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Recombinant human PD-L1, amino acid (Phe19-Arg238) (Accession: #Q9NZQ7.1), with a C-terminal 6x His, was expressed in 293E cells.
Molecular Mass
The 226 amino acid recombinant protein has a predicted molecular mass of approximately 26.01 kD. The DTT-reduced and non-reduced protein migrates at approximately 32-40 kD by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity
> 98%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human PD-1 (Cat. No. 789402) binds to immobilized recombinant human PD-L1 in a dose-dependent manner. Recombinant human PD-L1 was immobilized using purified anti-His Tag antibody (Cat. No. 652501). HRP-Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.6 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell

Function
Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes
Ligand/Receptor
PD-1 (CD279)
Bioactivity
Tested by ability of PD-L1 to bind to its ligand PD-1
Cell Type
Antigen-presenting cells, Dendritic cells, Monocytes
Biology Area
Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Dong H, et al. 1999. Nat Med. 5:1365-9.
  2. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34.
  3. Latchman Y, et al. 2001. Nat Immunol. 2:261-8. 
  4. Latchman YE, et al. 2004. Proc Natl Acad Sci USA. 101:10691-6. 
  5. Guleria I, et al. 2005. J Exp Med. 202:231-7.
  6. Lin DY, et al. 2008. Proc Natl Acad Sci USA. 105:3011-6.
  7. Dai S, et al. 2014. Cell Immunol. 290:72-9. 
  8. Melero I, et al. 2015. Nat Rev Cancer. 15:457-72. 
Gene ID
29126 View all products for this Gene ID
UniProt
View information about PD-L1 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 04/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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