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- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, FN14, TWEAKR, CD266.
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human TNFRSF12A inhibits the proliferation induced by TNFSF12 (TWEAK) in HUVEC cells. -
Human TNFRSF12A was aliquoted in PBS, pH 7 at 0.2 mg/ml and one aliquot was kept at 4°C (control), and another was freeze/thawed four times (4x freeze/thaws). After this procedure, the samples were tested for their ability to inhibit the proliferation of HUVEC cells induced by TNFSF12 (TWEAK).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 769104 | 25 µg | £150 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TNFRSF12A was initially identified as a fibroblast growth factor-1-inducible gene in murine NIH 3T3 cells, and was named as fibroblast growth factor-inducible-14 (Fn14) gene. Human TNFRSF12A was cloned from a HUVEC cDNA library and identified as the TWEAK receptor and a member of the TNFR family. It is a type-I transmembrane protein of 128 amino acids in length and has a cysteine rich region in the extracellular domain and a TRAF-binding motif in the intracellular domain. TNFRSF12A binds to TRAF2 (TRAFs 1, 3, and 5) in yeast two-hybrid screen and can initiate both canonical and noncanonical NF-κB activation. Also, TNFRSF12A-TNFRSF12 signaling induces lysosomal degradation of a cIAP1–TRAF2 complex to sensitize tumor cells to TNFRSF12A and its ligand trigger proliferation of mesenchymal progenitor cells and inhibit differentiation of chondrocytes, osteoblasts, and myocytes. TNFRSF12A expression is upregulated on epithelial and mesenchymal progenitor cells after tissue damage induced by including hypoxia, oxidative stress, chemical and mechanical injuries, inflammation, and tumor growth. TNFRSF12A and its ligand play a key role in muscle atrophy, cerebral ischemia, kidney injury, atherosclerosis, experimental autoimmune encephalitis, rheumatoid arthritis, and inflammatory bowel disease.
Product DetailsProduct Details
- Source
- Human TNFRSF12A, amino acids (Glu28-Trp79) (Accession # NP_057723) was expressed in 293E cells. Human IgG-Fc is in the carboxyl-terminus.
- Molecular Mass
- The 291 amino acid recombinant protein has a predicted molecular mass of approximately 32.4kD. The DTT-reduced and non-reduced protein migrate at approximately 40 kD and 80 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Endotoxin Level
- Less than 0.10 EU per µg of protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human TNFRSF12A inhibits the HUVEC cell proliferation induced by human TWEAK at 15 ng/ml. The ED50 = 10 - 50 ng/ml.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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Endothelial and epithelial cells, smooth muscle cells, fibroblasts, highly expressed on tumor cell lines of non-lymphoid origin.
- Function
- Endothelial cell migration, proliferation, and angiogenesis, regulation in wound healing.
- Interaction
- Macrophages, activated monocytes, and T cells.
- Ligand/Receptor
- TNFSF12, TWEAK, CD255
- Bioactivity
- Inhibits HUVEC cell proliferation induced by recombinant human TWEAK.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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1. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
2. Wiley SR, et al. 2001. Immunity. 15:837.
3. Brown SA, et al. 2003. Biochem. J. 371:395.
4. Saitoh T, et al. 2003. J. Biol. Chem. 278:36005.
5. Girgenrath M, et al. 2006. EMBO J. 25:5826.
6. Vince JE, et al. 2008. J. Cell. Biol. 182:171.
7. Wajant H, 2013. Br. J. Pharmacol. 170: 748.
8. Fick A, et al. 2012. J. Biol. Chem. 287:484.
9. Burkly LC, 2014. Semin. Immunol. 26:229. - Gene ID
- 51330 View all products for this Gene ID
- UniProt
- View information about TNFRSF12A on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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