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Recombinant Mouse IL-2 (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
IL2
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Product Citations
publications
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575409 4 pack £65
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Technical Data Sheet (pdf)

Product Details

Purity
>98%, as determined by SDS-PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA
Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 2 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-2 protein is useful as a standard for a mouse IL-2 sandwich ELISA, using unlabeled JES6-1A12 antibody (catalog # 503702) for capture and biotinylated JES6-5H4 antibody (catalog # 503804) for detection.

Product Citations
  1. Elazar A, et al. 2022. Elife. 11:. PubMed
  2. Høydahl LS, et al. 2021. Vaccine. 39:1583. PubMed
  3. Li H, et al. 2019. Investig Clin Urol. 60:359. PubMed

Antigen Details

Cell Sources
Mouse IL-2, amino acids Ala 21-Gln 169 (Accession # NM_008366) was expressed in E. coli.
Reconstitution
Sterile phosphate-buffered saline is recommended.
Biology Area
Stem Cells, Immunology, Cell Biology
Molecular Family
Cytokines/Chemokines
Gene ID
16183 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org
Go To Top Version: 2    Revision Date: 02/29/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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