Recombinant Rat IL-6 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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IL-6_Rat_RECOM_CF_1_090424
Recombinant rat IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.6 – 6 pg/mL.
  • IL-6_Rat_RECOM_CF_1_090424
    Recombinant rat IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.6 – 6 pg/mL.
  • IL-6_Rat_RECOM_CF_2_090424
    Stability Testing for Recombinant Rat IL-6. Recombinant rat IL-6 was aliquoted in 0.1% trifluoro acetic acid, 10% Acetonitrile and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property induce the proliferation of of mouse 7TD1 cells in a dose-dependent manner. The ED50 for this effect is 0.6 – 6.0 pg/mL.
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760002 10 µg £101
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760004 25 µg £182
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Description

IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. IL-6 was initially identified as a B-cell differentiation factor, capable of inducing the maturation of B cells into antibody-producing cells. IL-6 is the major circulating pyrogen during inflammation or injury. Several studies suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (–/–) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause the proliferation and migration of systemic endothelial cells in culture. The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80 kD cognate-chain (IL-6R), and a ubiquitously expressed 130 kD β-chain receptor (gp130), which acts as the universal signal-transducing element for all IL-6 family cytokines. Alternatively, IL-6 regulation of leukocyte trafficking relies upon the signaling via its soluble IL-6R (termed IL-6 trans-signaling). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production. IL-6 is a key factor that reciprocally regulates Th17 and FOXP3(+) Treg differentiation by the inhibition of TGF-β induced FOXP3 and induction of RORγt, which is a Th17 lineage-specific transcription factor.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Rat IL-6, amino acids Met-(Phe25-Thr211) (Accession# NM_012589), was expressed in E. coli.
Molecular Mass
The 188 amino acid recombinant protein has a predicted molecular mass of approximately 21.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 22 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant rat IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.6 – 6 pg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Growth factor.
Distribution

IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.

Function
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, and IL-17 and is downregulated by glucocorticoids IL-4 and TGF-β.
Interaction
T cells, B cells, hepatocytes, and cholinergic neurons.
Ligand/Receptor
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. McClintock JY, Wagner EM. 2005. J. Appl. Physiol. 99:861.
2. Murakami M, et al. 1993. Science 260:1808.
3. Cartmell T, et al. 2000. J. Physiol. 526:653.
4. Jones SA, et al. 2001. FASEB J. 15:43.
5. Fielding CA, et al. 2008. J. Immunol. 181:2189.
6. Sonderegger I, et al. 2008. Eur. J. Immunol. 38:1833.
7. Erta M, et al. 2012. Int. J. Biol. Sci. 8:1254.
8. Rosean TR, et al. 2014. Immunol. Res. 59:188.

Gene ID
24498 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 3    Revision Date: 09/04/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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