Spark Blue™ 574 anti-mouse CD279 (PD-1) (Flexi-Fluor™) Antibody, CD279, 29F.1A12

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Clone: 29F.1A12 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, Programmed Death-1, PDCD1
Isotype: Rat IgG2a, κ
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Product Citations: publications

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285153

50 µg

£230

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Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue³, in vivo blocking of PD-1 binding to its ligands^2,3, and spatial biology (IBEX)^5,6.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662493 (BioLegend Cat. No. 285153)

Antigen Details

Structure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
Function: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, T cells
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313
Gene ID: 18566 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All PD-1 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD279 (PD-1)	29F.1A12	FC
Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block,ELISA
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)	29F.1A12	FC
APC anti-mouse CD279 (PD-1)	29F.1A12	FC
Biotin anti-mouse CD279 (PD-1)	29F.1A12	FC
FITC anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 421™ anti-mouse CD279 (PD-1)	29F.1A12	FC,SB
Brilliant Violet 605™ anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 785™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Dazzle™ 594 anti-mouse CD279 (PD-1)	29F.1A12	FC
Alexa Fluor® 647 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 711™ anti-mouse CD279 (PD-1)	29F.1A12	FC
GoInVivo™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Fire™ 750 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 510™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block
APC/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Cyanine5 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 640 anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Red™ 718 anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 806 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 750™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 780 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 700 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 650™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Blue™ 574 anti-mouse CD279 (PD-1) (Flexi-Fluor™)	29F.1A12	FC
Spark PLUS B550™ anti-mouse CD279 (PD-1)	29F.1A12	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.