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Spark Blue™ 574 Streptavidin

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Regulatory Status
RUO
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Streptavidin_SparkBlue574_Antibody_050423
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Blue™ 574 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Blue™ 574 and anti-human CD19 APC (right).
  • Streptavidin_SparkBlue574_Antibody_050423
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Blue™ 574 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Blue™ 574 and anti-human CD19 APC (right).
See Spark Blue™ 574 spectral data
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405355 25 µg £73
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405356 100 µg £174
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark Blue™ 574 under optimal conditions
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin- Spark Blue™ 574 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this Streptavidin-Spark Blue™ 574 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Blue™ 574 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.

Excitation Laser
Blue Laser (488 nm)

Related FAQs

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Go To Top Version: 1    Revision Date: 04/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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