- Clone
- S21010F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Leu2, CD8 Antigen
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
388805 | 25 tests | £76 | ||||
388806 | 100 tests | £189 |
CD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK Cells, as well as a majority off thymocytes. CD8α can form a homodimer (CD8α/α) or heterodimer (CD8α/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD8α-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1.
Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40.
Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
Subset of T Cells, most thymocytes, NK cells
- Function
- TCR co-receptor
- Interaction
- CD8β
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Leahy, D.J, 1995. FASEB J. 9:17-25.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8alpha on UniProt.org
Related FAQs
Other Formats
View All CD8a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD8a | S21010F | FC |
PE anti-human CD8a | S21010F | FC |
Spark Red™ 718 anti-human CD8a | S21010F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD8a
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us