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Spark Red™ 718 anti-human CD8a Antibody

Pricing & Availability
Clone
S21010F (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2, CD8 Antigen
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
S21010F_Spark-Red-718_CD8a_061024
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human CD8a (clone S21010F) Spark Red™ 718 (left) or mouse IgG2b, κ Spark Red™ 718 isotype control (right).
  • S21010F_Spark-Red-718_CD8a_061024
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human CD8a (clone S21010F) Spark Red™ 718 (left) or mouse IgG2b, κ Spark Red™ 718 isotype control (right).
Compare all formats See Spark Red™ 718 spectral data
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388805 25 tests £76
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388806 100 tests £189
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Description

CD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK Cells, as well as a majority off thymocytes. CD8α can form a homodimer (CD8α/α) or heterodimer (CD8α/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD8α-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1.

Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40.

Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.

Antigen Details

Structure
Ig superfamily
Distribution

Subset of T Cells, most thymocytes, NK cells

Function
TCR co-receptor
Interaction
CD8β
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
  2. Leahy, D.J, 1995. FASEB J. 9:17-25.
Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 1    Revision Date: 06/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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