TotalSeq™-D0151 anti-human CD152 (CTLA-4) antibody, CD152, BNI3

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Clone: BNI3 (See other available formats)
Regulatory Status: RUO
Other Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype: Mouse IgG2a, κ
Barcode Sequence: ATGGTTCACGTAATC
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Product Citations: publications

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369635

10 µg

£253

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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2894575 (BioLegend Cat. No. 369635)

Antigen Details

Structure: Ig superfamily and 33 kD.
Distribution: Activated T cells and B cells.
Function: Negative regulator of T cell activation.
Interaction: Antigen presenting cells, such as dendritic cells.
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86).
Cell Type: B cells, T cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.
Gene ID: 1493 View all products for this Gene ID
UniProt: View information about CD152 on UniProt.org

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All CD152 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 421™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 605™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Biotin anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine7 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-A0151 anti-human CD152 (CTLA-4)	BNI3	PG
TotalSeq™-C0151 anti-human CD152 (CTLA-4)	BNI3	PG
Brilliant Violet 785™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-B0151 anti-human CD152 (CTLA-4)	BNI3	PG
APC/Fire™ 750 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Alexa Fluor® 647 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody	BNI3	ICFC,FC
Brilliant Violet™ 711 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-D0151 anti-human CD152 (CTLA-4)	BNI3	PG
PE/Fire™ 640 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Red™ 718 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 744 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 810 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 650™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 510™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Blue™ 574 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC
Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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