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Treg-Protector™ (anti-ARTC2 Nanobody)

Pricing & Availability
Clone
S+16a (See other available formats)
Regulatory Status
RUO
Other Names
ART2.2, Art2b, Art, Rt6, Rt-6, Ly92b, Rt6-2
Isotype
Llama VH Ig
Ave. Rating
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Product Citations
publications
S-16a_Treg-Protector_Block_1_020215
A C57BL/6 mouse was injected (i.v.) with 50 µg of Treg-Protector™ 15 minutes before harvesting the spleen to isolate lymphocytes (top). The same procedure was done with a control mouse that only received PBS (bottom). The cells obtained from each mouse were incubated for one hour at 37°C and then were stained with CD3 Brilliant Violet 605™, CD4 FITC, CD25 PE/Dazzle™ 594, and Annexin V Brilliant Violet 421™. Data shown was gated on the CD3+, and CD4+ population. Note the higher frequency of live Tregs (CD4+, CD25+, and Annexin V- T cells) in the mouse that was treated with Treg-Protector™ (top).
  • S-16a_Treg-Protector_Block_1_020215
    A C57BL/6 mouse was injected (i.v.) with 50 µg of Treg-Protector™ 15 minutes before harvesting the spleen to isolate lymphocytes (top). The same procedure was done with a control mouse that only received PBS (bottom). The cells obtained from each mouse were incubated for one hour at 37°C and then were stained with CD3 Brilliant Violet 605™, CD4 FITC, CD25 PE/Dazzle™ 594, and Annexin V Brilliant Violet 421™. Data shown was gated on the CD3+, and CD4+ population. Note the higher frequency of live Tregs (CD4+, CD25+, and Annexin V- T cells) in the mouse that was treated with Treg-Protector™ (top).
  • S-16a_Treg-Protector_Block_2_020215
See supplemental data
Cat # Size Price Quantity Check Availability Save
149801 250 µg £238
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149803 500 µg £318
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149802 1 mg £558
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Description

ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Immunogen
Expression vector encoding full length mouse ARTC2.
Formulation
1 mg/mL S+16a Nanobody in PBS. Purity ≥ 95%, as determined by Coomassie stained SDS-PAGE.
Endotoxin Level
Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.
Preparation
Treg-Protector ™ is sterilized by filtration through a 0.22 µm membrane and contains no preservatives.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C. Do not freeze. Keep sterile.
Application

Block - Quality tested
Recommended Usage

We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).

Application Notes

Treg-Protector™ (S+16a Nanobody) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a nanobody has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells8.

Application References

(PubMed link indicates BioLegend citation)
  1. Rissiek B, et al. 2014. J. Leuko. Biol. 95:543.
  2. Bannas P, et al. 2014. Contrast Media Mol. Imaging 9:135.
  3. Lischke T, et al. 2013. Eur. J. Immunol. 43:1828.
  4. Hubert S, et al. 2010. J. Exp. Med. 207:2561.
  5. Scheuplein F, et al. 2010. J. Autoimmun. 34:145.
  6. Scheuplein F, et al. 2009. J. Immunol. 182:2898.
  7. Koch-Nolte F, et al. 2007. FASEB J. 21:3490.
  8. Fernandez-Ruiz D, et al. 2016. Immunity. 45:889.
Product Citations
  1. Wanhainen KM, et al. 2022. Cancer Immunol Res. 10:871. PubMed
  2. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  3. Künzli M, et al. 2020. Sci Immunol. :5. PubMed
  4. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  5. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  6. Liu Q, et al. 2019. J Immunol. 203:1189. PubMed
RRID
AB_2565485 (BioLegend Cat. No. 149801)
AB_2565485 (BioLegend Cat. No. 149803)
AB_2565485 (BioLegend Cat. No. 149802)

Antigen Details

Structure
GPI-anchored surface protein, 35 kD
Distribution

Subsets of mature T cells, regulatory T cells (Tregs), natural killer T cells (NKTs), and γδ T cells.

Function
ADP-ribosyltransferase.
Interaction
P2X7
Cell Type
NKT cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References

1. Menzel S, et al. 2013. FEBS J. 280:3543.
2. Bannas P, et al. 2011. Mol. Immunol. 48:1762.
3. Chen YG, et al. 2011. J. Immunol. 186:4278.
4. Adriouch S, et al. 2007. J. Immunol. 179:186.
5. Bannas P, et al. 2005. Blood 105:3663.

Gene ID
11872 View all products for this Gene ID
UniProt
View information about ARTC2 on UniProt.org
Go To Top Version: 2    Revision Date: 10/27/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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