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Ultra-LEAF™ Purified anti-human CD270 (HVEM) Antibody

Pricing & Availability
Clone
W22113E (See other available formats)
Regulatory Status
RUO
Other Names
Herpes virus entry mediator (HVEM), TNFRSF14, ATAR, CD270, Herpesvirus entry mediator A, Tumor necrosis factor receptor superfamily, member 14,
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22113E_ULEAF_CD270_Block_031524
Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794906) (black circles) binds to immobilized human HVEM-Fc chimera (Cat. No. 596506). Ultra-LEAF™ purified anti-human CD270 (HVEM) (clone W22113E) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. This antibody blocks the binding of human BTLA-Fc chimera to 0.1 µg/mL immobilized recombinant human HVEM-Fc chimera with ND50 range: 18 – 110 ng/mL.
  • W22113E_ULEAF_CD270_Block_031524
    Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794906) (black circles) binds to immobilized human HVEM-Fc chimera (Cat. No. 596506). Ultra-LEAF™ purified anti-human CD270 (HVEM) (clone W22113E) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. This antibody blocks the binding of human BTLA-Fc chimera to 0.1 µg/mL immobilized recombinant human HVEM-Fc chimera with ND50 range: 18 – 110 ng/mL.
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636153 100 µg £288
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636154 1 mg £729
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Description

Herpes virus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor family, mediates herpes virus entry into cells during infection. Upon overexpression, HVEM activates NF-κB and AP-1 through a TNF receptor-associated factor (TRAF)-mediated mechanism. HVEM ligands belong to two distinct families: the TNF-related cytokines LIGHT and lymphotoxin-α, and the Ig-related membrane proteins BTLA and CD160. HVEM and its ligands have been involved in the pathogenesis of various autoimmune and inflammatory diseases, tumor progression and resistance to immune response. It has been demonstrated that HVEM induces a novel signaling pathway downstream leading to signal transduction and activation of STAT3 in epithelial cells. Since STAT3 regulates the expression of genes important for host defense in epithelial cells, as well as the differentiation of retinoid-related orphan receptor RORγt+, Th17, and innate lymphoid cells. This finding suggests that HVEM may play an important role in mucosal host defense.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human HVEM
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.8 μg/mL Recombinant Human BTLA-Fc Chimera to 0.1 μg/mL immobilized human HVEM-Fc Chimera. ND50 range: 18 – 110 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662317 (BioLegend Cat. No. 636153)
AB_3662317 (BioLegend Cat. No. 636154)

Antigen Details

Distribution

Spleen, thymus, lung, macrophages, B cells and T cells

Function
HVEM plays a role in mucosal host defense, autoimmune and inflammatory diseases, and tumor progression
Interaction
T cells, NK cells
Ligand/Receptor
LIGHT, lymphotoxin-alpha, BTLA, CD160, TRAF proteins
Cell Type
Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Pasero C, et al. 2012. Curr Opin Pharmacol. 12:478-85.
  2. Hsu H, et al. 1997. J Biol Chem. 272:13471-4.
  3. Mauri DN, et al. 1998. Immunity. 8:21-30.
  4. Montgomery RI, et al. 1996. Cell. 87:427-36.
  5. Shui JW, et al. 2012. Nature. 488:222-5.
Gene ID
8764 View all products for this Gene ID
UniProt
View information about CD270 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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