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- Clone
- W22247A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Teratoma-derived growth factor 1 (TDGF-1), Epidermal Growth Factor-Like Cripto Protein (CR1), Cripto-1 Growth Factor, CR, CRGF, CR-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human Cripto (carrier-free) (Cat. No. 777804) (black circles) binds to immobilized recombinant human Activin R1B/ALK-4-Fc chimera (Cat. No. 770406). Ultra-LEAF™ purified anti-human Cripto (TDGF-1) (clone W22247A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the purified rat IgG2a, κ isotype control (Cat. No. 400502) (gray triangles) does not have the effect. This antibody blocks the binding of recombinant human Cripto to 1 µg/mL immobilized recombinant human Activin R1B/ALK-4-Fc chimera with ND50 range: 0.8 – 5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 636053 | 100 µg | £295 | ||||
| 636054 | 1 mg | £777 | ||||
Cripto is a glycosylphosphatidylinositol (GPI)-anchored Type I cell membrane protein that belongs to the epidermal growth factor (EGF)-Cripto-1-FRL-1-Cryptic (CFC) family of proteins (EGF-CFC). In addition to Cripto, another three members of the EGF-CRC family have been described: Criptic, Xenopus FRL-1, and zebrafish one-eyed pinhead (0EP). Cripto contains a signal sequence, an EGF-like domain, a cysteine-rich region motif (CFC domain), and a hydrophobic COOH-terminus. Cripto plays an important role in maintaining embryonic stem cell pluripotency and tumorigenesis. Cripto acts as a co-receptor for Nodal, a TGFb family member that plays a crucial role in mesoderm and endoderm formation. Nodal and Cripto form complex with type I Activin receptors, Alk4/7 (ACVR1B/ACVR1C), and type II Activin receptors, ActRIIA/ActRIIB (ACVR2A/ACVR2B). Cripto is also known to bind Furin and Pace4 that process Nodal proteolytic maturation and supports Nodal signaling. A soluble form of Cripto is secreted by tumor cells and it lacks the GPI anchor region which is removed by GPI-phospholipase D. The soluble Cripto promotes endothelial cell migration and possibly tumor angiogenesis. More recent data showed that a GPI phospholipase A2 called post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6) in involved in the in the shedding of Cripto. PGAP6 produces Cripto bearing lyso-GPI which is released from the membrane, and subsequently Cripto is released from the lyso–GPI by GPI-phospholipase D.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human Cripto
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by blocking the binding of 450 ng/mL Recombinant Human Cripto (carrier-free) to 1 μg/mL immobilized Recombinant Human Activin R1B/ALK-4-Fc Chimera (carrier-free). ND50 range: 0.8-5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Distribution
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Mainly expressed during embryogenesis, embryonic stem cells, induced in pluripotent stem cells. Low expression in adult tissues, hematopoetic stem cells, mammary epithelial cells, adipose tissue, pancreas, and endometrium.
- Function
- Induces cell growth, migration, invasion, and epithelial to mesenchymal transition. Participates in tumorigenesis, cancer cell proliferation and survival.
- Interaction
- Interacts with Nodal, ALK4, Furin, PACE4, and GRP78
- Ligand/Receptor
- Co-receptor of Nodal, GDF1, and GDF3
- Biology Area
- Cancer Biomarkers, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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- Ciccodicola A, et al. 1989. EMBO J. 8:1987-91.
- Beck S, et al. 2002. Nature Cell Biology. 4:981-5.
- Gray PC, et al. 2003. Proc Natl Acad Sci USA. 100:5193-8.
- Xing PX, et al. 2004. Cancer Res. 64:4018-23.
- Watanabe K, et al. 2007. J Biol Chem. 282:35772-86.
- Blanchet MH, et al. 2008. EMBO J. 27:2580-91.
- Gray PC and Vale W. 2012. FEBs Letter. 586:1836-45.
- Lee GH, et al. 2016. J Cell Biol. 215:705-718.
- Zoni E, et al. 2017. Oncogene. 36:4739-4749.
- Gene ID
- 6997 View all products for this Gene ID
- UniProt
- View information about Cripto on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Cripto Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human Cripto (TDGF-1) | W22247A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human Cripto (TDGF-1)
Recombinant human Cripto (carrier-free) (Cat. No. 777804) (b...
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