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Ultra-LEAF™ Purified anti-human CD48 Antibody

Pricing & Availability
Clone
W20019A (See other available formats)
Regulatory Status
RUO
Other Names
CD48 antigen, B-lymphocyte activation marker BLAST-1, Blast-1, SLAMF2, HuLy-m3, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20019A_ULEAF_CD48_Antibody_011322
Biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc Chimera (black circles) binds to immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). Ultra-LEAF™ purified anti-human CD48 (clone W20019A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc Chimera. ND50 range: 0.04 - 0.2 µg/mL
  • W20019A_ULEAF_CD48_Antibody_011322
    Biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc Chimera (black circles) binds to immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). Ultra-LEAF™ purified anti-human CD48 (clone W20019A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc Chimera. ND50 range: 0.04 - 0.2 µg/mL
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947103 100 µg £148
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947104 1 mg £591
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD48, also known as SLAMF2, is one of the members of the CD2 subfamily. CD48 is a GPI-anchored membrane protein containing two Ig-like C2 type domains and is widely expressed on both resting and activated hematopoietic cells with the exception of granulocytes, platelets, and erythrocytes. CD48 surface expression is elevated upon viral infection. Engagement of SLAMF4 (CD244) by a natural ligand, CD48, leads to cell-mediated cytotoxicity and IFN-γ secretion by NK cells via the adaptor protein, SAP. While CD48-expressing tumors were efficiently eliminated by NK cells in SLAMF4-deficient mice, indicating that the CD48/SLAMF4 interaction plays an inhibitory role on NK cells in a context-dependent manner. In mice lacking expression of self-MHC-binding inhibitory receptors, ligation of CD48 and SLAMF4 inhibits NK cell activation, indicating that CD48/SLAMF4 contributes to NK-cell self-tolerance in a MHC-I-independent manner. Differential expression of CD48 in distinct subsets of human dendritic cells affects NK cell activation. In addition, CD48 interacts with SLAMF4 and functions as a costimulatory molecule, playing a critical role in cell proliferation and MHC-restricted cytotoxicity in CD8+ T cells. CD48 on the surface of marginal zone B cells activates NK cells and stimulates Th2 cytokine IL-13 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD48 (SLAMF2)-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). ND50 range: 0.04 - 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2910509 (BioLegend Cat. No. 947103)
AB_2910509 (BioLegend Cat. No. 947104)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Widely expressed on hematopoietic cells except granulocytes, platelets, and erythrocytes. Increased on T and B cells after activation

Function
Contributes to T cell antigen recognition, plays an inhibitory and activating role on NK cells
Interaction
NK cells, subsets of T cells; Lck, Fyn interact with cytoplasmic domain
Ligand/Receptor
SLAMF4 (CD244)
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Veillette A. 2004. J Exp Med. 199:1175-8.
  2. Fisher RC and Thorley-Lawson DA. 1991. Mol Cell Biol. 11:1614-23.
  3. Yokoyama S, et al. 1991. J Immunol. 146:2192-200.
  4. Lee KM, et al. 2004. J Exp Med. 199:1245-54.
  5. Assarsson E, et al. 2005. J Immunol. 175:2045-9.
  6. Korinek V, et al. 1991. Immunogenetics. 33:108-12. 
  7. Knapp W, et al. 1989. Immunol Today. 10:253-8. 
  8. Schlossman S, et al. 1995. Oxford University Press
  9. McNerney ME, et al. 2005. Blood. 106:1337-40. 
  10. Gao N, et al. 2006. J Immunol. 176:2758-64.
  11. Morandi B, et al. 2005. J Immunol. 175:3690-7.
  12. Messmer B, et al. 2006. J Immunol. 176:4646-50.
  13. Lee KM, et al. 2003. J Immunol. 170:4881-5.
  14. Kato K, et al. 1992. J Exp Med. 176:1241-9.
  15. Lee KM, et al. 2006. Blood. 107:3181-8. 
Gene ID
962 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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