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Ultra-LEAF™ Purified anti-human B7-H6 Antibody

Pricing & Availability
Clone
W20205G (See other available formats)
Regulatory Status
RUO
Other Names
B7H6, NCR3LG1, natural killer cell cytotoxicity receptor 3 ligand 1, B7 Homolog 6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20205G_ULEAF_B7H6_Antibody_102722
Biotinylated recombinant human NKp30 (black circles) binds to immobilized recombinant human B7-H6-Fc Chimera (Cat. No. 794006). Ultra-LEAF™ purified anti-human B7-H6 (clone W20205G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 1 µg/mL biotinylated recombinant human NKp30 to 1 µg/mL immobilized recombinant Human B7-H6-Fc Chimera. ND50 range: 0.01 - 0.08 µg/mL.
  • W20205G_ULEAF_B7H6_Antibody_102722
    Biotinylated recombinant human NKp30 (black circles) binds to immobilized recombinant human B7-H6-Fc Chimera (Cat. No. 794006). Ultra-LEAF™ purified anti-human B7-H6 (clone W20205G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 1 µg/mL biotinylated recombinant human NKp30 to 1 µg/mL immobilized recombinant Human B7-H6-Fc Chimera. ND50 range: 0.01 - 0.08 µg/mL.
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605953 100 µg £223
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Description

B7-H6 is a type I transmembrane protein and member of the B7 family. It contains 454 amino acids with a predicted molecular weight of 51 kD and has several potential N-glycosylation sites. Similar to the other members of the B7 family, B7-H6 possesses two Ig like domains in the extracellular domain. The most related proteins to B7-H6 are B7-H1 and B7-H3. B7-H6 is expressed in human cancer cells such as myeloid and lymphoblastic leukemia, neuroblastomas, and melanomas, and it binds to NKp30 to activate human NK cells and target cell killing. Nevertheless, tumor cells can escape NK-cell-mediated recognition. A mechanism of immune escape involves tumor cell shedding of B7-H6. That process is mediated by ADAM10 and ADAM17. Thus, soluble B7-H6 high levels in blood have been detected in patients with malignant melanoma; levels that correlate with elevated B7-H6 expression in melanoma biopsies. In addition, B7-H6 is expressed in glioma stem-like cells derived from glioma cell lines in vitro. Soluble isoforms form of B7-H6 have also been detected in serum during normal pregnancy. These isoforms, of ∼37kD and ∼30kD, maintain the C- and N-terminus, and it seems that they are released by a mechanism different from proteolytic cleavage. B7-H6 can be induced in healthy cells by inflammatory process. B7-H6 and NKp30 orthologues do not exist in mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human B7-H6
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1 µg/mL biotinylated recombinant human NKp30 to 1 µg/mL immobilized recombinant human B7-H6 (Cat. No. 794006). ND50 range: 0.01 - 0.08 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927840 (BioLegend Cat. No. 605953)

Antigen Details

Structure
Dimer
Distribution

Human tumor cell lines, cancer cells, monocytes and neutrophils after inflammatory stimulation, glioma stem-like cells. Not detected in healthy cells.

Function
Natural killer (NK) cell activation and cytotoxicity
Interaction
NK cells
Ligand/Receptor
NKp30 (CD337)
Cell Type
Leukemia, Monocytes, Neutrophils
Biology Area
Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology
Antigen References
  1. Brant CS, et al. 2009. J Exp Med. 206:1495-503.
  2. Kaifu T, et al. 2011. Cell Mol Life Sci. 68:3531-9.
  3. Matta J, et al. 2013. Blood. 122:394-404.
  4. Schlecker E, et al. 2014. Cancer Res. 74:3429-40.  
  5. Chen Y, et al. 2018. Path Oncology Res. 24:717-21.
  6. Gutierrez-Franco J, et al. 2018. Immunobiology. 223:57-63.
  7. Obiedat A, et al. 2020. J Mol Med. 98:135-48.
  8. Chen H, et al. 2020. J Immunol Res. 2020:2328675.
Gene ID
374383 View all products for this Gene ID
UniProt
View information about B7-H6 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/07/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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