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Ultra-LEAF™ Purified anti-human BAFF (CD257) Antibody

Pricing & Availability
Clone
W20066D (See other available formats)
Regulatory Status
RUO
Other Names
B cell activating factor belonging to the TNF family (BAFF), B lymphocytes stimulator (BLyS), TNF- and APOL-related leucocyte expressed ligand 1(TALL1), TNF homolog that activates apoptosis, NKFB, JNK (THANK), TNF13B, CD257
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W20066D_ULEAF_BAFF_NEUT_061124
Recombinant Human BAFF (Cat. No. 559602) (black circles) induces the proliferation of IgM-stimulated primary mouse B cells. Ultra-LEAF™ Purified Anti-Human BAFF (clone W20066D) (purple squares) neutralizes proliferation induced by Recombinant Human BAFF at 6.7 ng/mL in a dose-dependent manner, whereas Ultra-LEAF™ Purified mouse IgG2b, κ isotype control (clone MG2b-57) (Cat. No. 401215) (gray triangles) does not have an effect. The ND50 is 4-24 ng/mL.
  • W20066D_ULEAF_BAFF_NEUT_061124
    Recombinant Human BAFF (Cat. No. 559602) (black circles) induces the proliferation of IgM-stimulated primary mouse B cells. Ultra-LEAF™ Purified Anti-Human BAFF (clone W20066D) (purple squares) neutralizes proliferation induced by Recombinant Human BAFF at 6.7 ng/mL in a dose-dependent manner, whereas Ultra-LEAF™ Purified mouse IgG2b, κ isotype control (clone MG2b-57) (Cat. No. 401215) (gray triangles) does not have an effect. The ND50 is 4-24 ng/mL.
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638353 100 µg £295
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638354 1 mg £777
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Description

BAFF is a TNF-α cytokine member (a type II membrane protein) that acts in both a membrane-bound form and soluble cytokine form. The extracellular domain of BAFF shows high homology with APRIL. The soluble form is derived by cleavage in a furin consensus site. In human neutrophils treated with G-CSF, BAFF is processed intracellularly by a furin-type convertase. BAFF acts mainly as a soluble trimer and binds to three receptors with decreasing affinity in the following order: BAFFR > TACI > BCMA. Mice deficient in BAFF or its receptor BAFFR exhibit an obstruction in B cell development after the transitional T1 stage and lack marginal zone (MZ) and conventional B2 B cells. BAFFR-deficient mice and humans (patients with common variable immunodeficiency, or CVID) develop severe B cell lymphopenia due to the arrest of B cell development at the transitional B cell stage. Extreme BAFF production triggers severe autoimmune disorders in mice similar to Systemic Lupus Erythematosus (SLE) and Sjögren’s syndrome. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, SLE, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin’s lymphoma (NHL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human BAFF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the proliferation of primary mouse B cells induced by recombinant human BAFF (Cat. Nos. 559602, 559604, 559606, and 559608) at a spike concentration determined from a standard curve. ND50 range: 4 - 24 ng/mL.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662320 (BioLegend Cat. No. 638353)
AB_3662320 (BioLegend Cat. No. 638354)

Antigen Details

Structure
Trimer
Distribution

Monocytes, macrophages, dendritic cells, neutrophils, and stromal cells of lymphoid organs

Function
BAFF induces B cell maturation, proliferation, survival, and immunoglobulin production. BAFF controls the size and composition of mature preimmune B cell pools. BAFF is induced by INF-γ in monocytes.
Interaction
Immature B cells, peripheral B-cells, naïve and memory cells
Ligand/Receptor
BAFFR, TACI (transmembrane activator and calcium-modulator and cyclophilin ligand interactor), and BCMA (B cell maturation antigen)
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References
  1. Schneider P, et al. 1999. J Exp Med. 189:1747-56.
  2. Nardelli B, et al. 2001. Blood. 97:198-204.
  3. Schiemann B, et al. 2001. Science. 5537:2111-4.
  4. Scapini P, et al. 2003. J Exp Med. 197:297-302.
  5. Yeramilli VA and Knight KL. 2010. J Immunol. 184:5527-36.
  6. Scholz JL and Cancro MP. 2012. Immunol. Lett. 143:2-8.
  7. Sindhava VJ, et al. 2013. Front Immunol. 4:37.
  8. Koizumi M, et al. 2013. PLoS One. 8:e71367.
Gene ID
10673 View all products for this Gene ID
UniProt
View information about BAFF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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