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Ultra-LEAF™ Purified anti-human BMP-6 Antibody

Pricing & Availability
Clone
A20139D (See other available formats)
Regulatory Status
RUO
Other Names
Bone morphogenetic protein 6, VGR1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A20139D_ULEAF_BMP-6_Antibody_062824
Recombinant human BMP-6 (Cat. No. 595502) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-humam BMP-6 (clone A20139D) (purple squares) inhibits the effect of recombinant human BMP-6 at 0.1 µg/mL in ATDC5 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 401507) (gray triangles) does not have effect. ND50 range: 0.1 - 0.6 µg/mL.
  • A20139D_ULEAF_BMP-6_Antibody_062824
    Recombinant human BMP-6 (Cat. No. 595502) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-humam BMP-6 (clone A20139D) (purple squares) inhibits the effect of recombinant human BMP-6 at 0.1 µg/mL in ATDC5 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 401507) (gray triangles) does not have effect. ND50 range: 0.1 - 0.6 µg/mL.
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643653 100 µg £295
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643654 1 mg £777
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Description

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human recombinant BMP-6 can restore bone in animal models of osteoporosis. BMP-6 induces bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. BMP-6 has been reported to have an antiproliferative effect on B, T cells, and macrophages. Also, BMP-6 induced expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. In addition, BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human BMP-6
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the differentiation induced by recombinant human BMP-6 (Cat. No. 595502) at 0.1 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 0.1 - 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662325 (BioLegend Cat. No. 643653)
AB_3662325 (BioLegend Cat. No. 643654)

Antigen Details

Structure
Growth factor, Homodimer
Distribution

Hepatocytes

Function
Osteoblast differentiation, bone regeneration. Antiproliferative effect on B, T cells, and macrophages. BMP-6 regulates hepcidin expression and iron metabolism in vivo.
Interaction
Cancer cells, mesenchymal stem cells germline stem cells, hematopoietic stem cells, and neural stem cells
Ligand/Receptor
BMP receptors Alk-2 and Alk-3 (type I), BMPRII (type II)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Chen D, et al. 2004. Growth Factors. 22:233-41.
  2. Grasser WA, et al. 2007. Int Orthop. 31:759-65.
  3. Sivertsen EA, et al. 2007. Eur J Immunol. 37:2937-48.
  4. Andriopoulos B, et al. 2009. Nat Genetics. 41:482-7. 
  5. Hong JH, et al 2009. Immunology. 128:e442-50.
  6. Lee GT, et al. 2011. Mol Immunol. 48:1540-7.
Gene ID
654 View all products for this Gene ID
UniProt
View information about BMP-6 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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