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Ultra-LEAF™ Purified anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
W18195E (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18195E_ULEAF_CD117_Antibody_1_062822
Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with Ultra-LEAF™ purified anti-human CD117 (c-kit) (clone W18195E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by PE goat anti-rat IgG.
  • W18195E_ULEAF_CD117_Antibody_1_062822
    Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with Ultra-LEAF™ purified anti-human CD117 (c-kit) (clone W18195E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by PE goat anti-rat IgG.
  • W18195E_ULEAF_CD117_Antibody_2_062822
    MO7E cells (a human megakaryocyte cell line) were pretreated with Ultra-LEAF™ purified anti-human CD117 (c-kit) (clone W18195E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) for 15 minutes, then 100 ng of Recombinant Human SCF (Cat. No. 573902) were added for another 5 minutes in 37°C incubation. Cell were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with APC anti-ERK1/2 Phospho (Thr202/Tyr204) (Cat. No. 369521).
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380203 100 µg £185
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD117 transfectant
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18195E can complete block binding of clones H18022H and W18195D on target cells.

Clone W18195E does not affect binding of clones 104D2, W18195C or S18022G on target cells.

Clone W18195E can block ligand binding on c-kit.

RRID
AB_2924570 (BioLegend Cat. No. 380203)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
Stem cell factor (c-kit ligand)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Giebel LB, et al. 1992. Oncogene. 7:2207-17.
  2. Furitsu T, et al. 1993. J Clin Invest. 92:1736-44.
Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/28/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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