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Ultra-LEAF™ Purified anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
W18063F (See other available formats)
Regulatory Status
RUO
Other Names
CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18063F_ULEAF_CD137_4-1BB_Antibody_071621.png
Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606) (black circles) binds to immobilized recombinant human TNFSF9 (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137 (4-1BB) antibody (clone W18063F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.03 µg/mL recombinant human TNFRSF9/4-1BB-Fc Chimera to 0.5 µg/mL of immobilized recombinant human TNFSF9 with an ND50 = 0.01 - 0.06 µg/mL.
  • W18063F_ULEAF_CD137_4-1BB_Antibody_071621.png
    Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606) (black circles) binds to immobilized recombinant human TNFSF9 (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137 (4-1BB) antibody (clone W18063F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.03 µg/mL recombinant human TNFRSF9/4-1BB-Fc Chimera to 0.5 µg/mL of immobilized recombinant human TNFSF9 with an ND50 = 0.01 - 0.06 µg/mL.
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944903 100 µg £245
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944904 1 mg £825
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducer of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps the optimal CD8+ T cell-mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD137
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between human TNFSF9 (Cat. No. 750006) and human TNFRSF9/4-1BB (Cat. No. 772606). The ND50 range: 0.01 - 0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Manes TD, et al. 2022. Front Immunol. 13:1016361. PubMed
RRID
AB_2904454 (BioLegend Cat. No. 944903)
AB_2904454 (BioLegend Cat. No. 944904)

Antigen Details

Structure
Dimer
Distribution

Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries

Function
Proliferation of T cells, activates their effector functions, survival, and establishes immunological memory
Interaction
Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells.
Ligand/Receptor
TNFSF9 and CD137L
Cell Type
B cells, Dendritic cells, Endothelial cells, Mast cells, T cells, Tregs
Biology Area
Immuno-Oncology, Immunology, Protein Trafficking and Clearance
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
  2. Watts TH. 2005. Annu. Rev. Immunol. 23:23-68.
  3. Zhu Y, et al. 2007. Blood 109:4882.
  4. Locatelli F. 2009. Blood 11:2208.
  5. Wang J, et al. 2010. J. Immunol. 185:7654.
  6. Eun SY, et al. 2015. J. Immunol. 1:134-41.
  7. Spencer AJ, et al. 2014. PLoS One. 9:e105520.
  8. Yi L, et al. 2014. PLoS One 1:e86337.
Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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