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Ultra-LEAF™ Purified anti-human CD137L (4-1BB Ligand) Antibody

Pricing & Availability
Clone
W22023G (See other available formats)
Regulatory Status
RUO
Other Names
CD137L, 4-1BB-L, 4-1BBL, 41BBL, 4-1BB Ligand, 41BB Ligand
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22023G_ULEAF_CD137L_Block_071524
Biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) (black circles) binds to immobilized recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137L (4-1BB Ligand) (clone W22023G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera to 1 µg/mL immobilized recombinant human TNFSF9 (4-1BBL). ND50 range: 0.01 - 0.07 µg/mL.
  • W22023G_ULEAF_CD137L_Block_071524
    Biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) (black circles) binds to immobilized recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137L (4-1BB Ligand) (clone W22023G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera to 1 µg/mL immobilized recombinant human TNFSF9 (4-1BBL). ND50 range: 0.01 - 0.07 µg/mL.
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647153 100 µg £312
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647154 1 mg £791
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Description

TNFSF9, also known as 4-1BBL and CD137L, is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC), and on B lymphocytes particularly. The interaction between TNFSF9 and its receptor, CD137, which is mainly expressed on T cells, leads to the activation of cytotoxic T cells. In addition, TNFSF9 (CD137) interaction can reactivate anergic T lymphocytes and induce T cell proliferation. This molecule plays a role in optimal CD8 T cell response. Interestingly, it appears that TNFSF9 is able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28. TNFSF9 signaling on T cells has also been shown to limit T cell activation. CD137L is upregulated in peripheral CD14+ monocytes of chronic hepatitis B patients and is associated with liver cirrhosis. Human TNFSF9 shares 36% of its amino acid sequence with its murine counterpart. Mice deficient of TNFSF9 are susceptible to develop B-cell lymphomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human TNFSF9 (4-1BBL)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) to immobilized 1 µg/mL recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006).  ND50 range: 0.01 - 0.07 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

 

RRID
AB_3662329 (BioLegend Cat. No. 647153)
AB_3662329 (BioLegend Cat. No. 647154)

Antigen Details

Structure
Monomer
Distribution

Constitutively expressed on a fraction of dendritic cells (DCs). Inducible on activated monocytes, macrophages, B cells, a small fraction of T cells, epithelial, and endothelial cells

Function
TNFSF9 plays roles in cytotoxic T cell activation, T cell proliferation, and apoptosis
Interaction
Activated T cells, natural killer cells (NK), macrophages, and dendritic cells
Ligand/Receptor
CD137
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Vinay DS and Kwon BS. 1998. Semin. Immunol. 10:481-9.
  2. Watts TH. 2005. Annu Rev Immunol. 23:23:23-68.
  3. Pollok KE, et al. 1994. Eur J Immunol. 24:367-74.
  4. Wang J, et al. 2010. J Immunol. 185:367-74.
  5. Eun SY, et al. 2014. J Immunol. 194:134.
  6. Spencer AJ, et al. 2014. PLoS One 9:e105520.
  7. Locatelli F. 2009. Blood. 114:2208-9.
Gene ID
8744 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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