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Ultra-LEAF™ Purified anti-human CD154 (CD40L) Antibody

Pricing & Availability
Clone
W21057A (See other available formats)
Regulatory Status
RUO
Other Names
CD154, TNF-related activator protein (TRAP), GP39, TNFSF5
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W21057A_ULEAF_CD154_Antibody_120823
Biotinylated recombinant CD40 (TNFRSF5)-Fc chimera (Cat. No. 777214) (black circles) binds to immobilized recombinant human CD40L (TNFSF5) (Cat. No. 591706). Ultra-LEAF™ purified anti-human CD154 (CD40L) (clone W21057A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG1, κ isotype control (Cat. No. 400432) (gray triangles) does not have effect. This antibody blocks the binding of 0.3 µg/mL Biotinylated human CD40 (TNFRSF5)-Fc chimera to 1 µg/mL immobilized recombinant human CD40L (TNFSF5). ND50 range: 0.024 - 0.15 µg/mL
  • W21057A_ULEAF_CD154_Antibody_120823
    Biotinylated recombinant CD40 (TNFRSF5)-Fc chimera (Cat. No. 777214) (black circles) binds to immobilized recombinant human CD40L (TNFSF5) (Cat. No. 591706). Ultra-LEAF™ purified anti-human CD154 (CD40L) (clone W21057A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG1, κ isotype control (Cat. No. 400432) (gray triangles) does not have effect. This antibody blocks the binding of 0.3 µg/mL Biotinylated human CD40 (TNFRSF5)-Fc chimera to 1 µg/mL immobilized recombinant human CD40L (TNFSF5). ND50 range: 0.024 - 0.15 µg/mL
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626653 100 µg £295
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626654 1 mg £575
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD40L is a type II membrane protein that shares 78% amino acid identity with its mouse counterpart. Human CD40L is a polypeptide of 261 amino acids, consisting of 22, 24, and 215 amino acids for the cytoplasmic, transmembrane, and extracellular domains respectively. Soluble forms of CD40L (sCD40L) have been identified, and an 18 kD fragment has the ability to form homotrimers and bind to CD40 to induce biological signals. The 18 kD protein has full activity in B cell proliferation and differentiation assays. Activated platelets express CD40L on their surface and constitute the major source of sCD40L (>95% in blood). Platelet-derived CD40L regulates neutrophil recruitment and lung damage in sepsis, and it has been suggested that MMP9 regulates CD40L shedding in abdominal sepsis. Platelet-associated CD40L has been linked to progression of atherosclerotic disease, and elevated plasma levels of sCD40L in patients seems to be a reliable predictor of cardiovascular events, such as atherosclerotic plaque rupture and acute coronary syndromes. Also, platelet-associated CD40L expression is increased in idiopathic thrombocytopenic purpura patients, and it has been speculated that platelet-associated-CD40L drives the activation of autoreactive B lymphocytes in this disease. In addition, high plasma levels of sCD40L have been connected to HIV-Associated Neurocognitive Disorders (HAND).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human CD40L (TNFSF5)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.3 µg/mL recombinant biotinylated human CD40 (TNFRSF5)-Fc (Cat. No. 777214) to immobilized 1 µg/mL recombinant human CD40L (TNFSF5) (Cat. No. 591706). ND50 = 0.024 - 0.15 µg/mL.

RRID
AB_3083417 (BioLegend Cat. No. 626653)
AB_3083417 (BioLegend Cat. No. 626654)

Antigen Details

Structure
Homotrimer
Distribution

T cells, basophils, mast cells, activated platelets

Function
CD40L induces proliferation, differentiation, and immunoglobulin class switching in B cells; also prevents apoptosis in B cell. CD40L is induced in T cells after in vitro activation with PHA, PMA plus ionomycin, anti-CD3, anti-CD2, and CD28 engagement in anti-CD3 activation. CD40L is also induced by thrombin in platelets.
Interaction
B cells, neutrophils, follicular dendritic cells, dendritic cells, macrophages, epithelial cells, epithelial carcinoma cells, endothelial cells, smooth muscle cells, fibroblasts, hepatic stellate cells, and glomerular mesangial cells
Ligand/Receptor
CD40
Biology Area
Cell Biology, Costimulatory Molecules
Molecular Family
Cytokines/Chemokines, Growth Factors, Immune Checkpoint Receptors
Antigen References
  1. Spriggs MK, et al. 1992. J Exp Med. 176:1543-50.
  2. Mazzei GJ, et al. 1995. J Biol Chem. 270:7025-8.
  3. Delmas Y, et al. 2005. Med. Sci. (Paris). 21:825-31.
  4. Meabed MH, et al. 2007. Haematology. 12:301.
  5. Sprangue DL, et al. 2008. Blood. 111:5028-36.
  6. Davidson DC, et al. 2012. PLoS One. 7: e51793.
  7. Rahman M, et al. 2013. J. Thromb. Haemost 11:1385-98.
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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