Ultra-LEAF™ Purified anti-human CD178 (FasL) Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

L5178Y mouse T lymphoma cells expressing recombinant human FasL

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, immunofluorescence microscopy³, immunocytochemistry², blocking of Fas induced apoptosis¹, and Western blotting¹¹. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).

Application References

(PubMed link indicates BioLegend citation)

1. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
2. Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
3. Bossi G, et al. 1999. Nature Medicine 5:90.
4. Andreola G, et al. 2002. J. Exp. Med. 195:1303.
5. Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
6. Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
7. Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
8. Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
9. Qin G, et al. 2012. J. Infect. Dis. PubMed
10. Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
11. Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
12. Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
13. Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
14. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
15. Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed

Product Citations

1. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
2. Dudek M, et al. 2021. Nature. 592:444. PubMed

RRID

AB_2810458 (BioLegend Cat. No. 306415)
AB_2810458 (BioLegend Cat. No. 306416)

Antigen Details

Structure

TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form

Distribution

Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells

Function

Apoptosis, immune privilege

Ligand/Receptor

CD95

Cell Type

Neutrophils, NK cells, T cells

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience

Molecular Family

CD Molecules

Antigen References

1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.

Gene ID

356 View all products for this Gene ID

UniProt

View information about CD178 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis