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Ultra-LEAF™ Purified anti-human CD20 (Rituximab Biosimilar, RUO) Recombinant Antibody

Pricing & Availability
Clone
QA20A02 (See other available formats)
Regulatory Status
RUO
Other Names
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, MS4A1, Leukocyte surface antigen Leu-16, membrane-spanning 4-domains superfamily A member 1
Isotype
Human IgG1, κ
Ave. Rating
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Product Citations
publications
A_QA20A02_ULEAF_CD20_Antibody_101122
Human peripheral blood lymphocytes were stained with Ultra-LEAF™ purified anti-human CD20 (Rituximab Biosimilar) recombinant (clone QA20A02) (left) or Ultra-LEAF™ purified human IgG1 isotype control (right), followed by PE anti-human IgG Fc recombinant. Cells were costained with anti-human CD19.
  • A_QA20A02_ULEAF_CD20_Antibody_101122
    Human peripheral blood lymphocytes were stained with Ultra-LEAF™ purified anti-human CD20 (Rituximab Biosimilar) recombinant (clone QA20A02) (left) or Ultra-LEAF™ purified human IgG1 isotype control (right), followed by PE anti-human IgG Fc recombinant. Cells were costained with anti-human CD19.
  • B_QA20A02_ULEAF_CD20_Antibody_2_122821
    Anti-human CD20 (Rituximab Biosimilar) Recombinant binds to human CD20 on the surface of Daudi cells activating complement- dependent cytotoxicity (CDC). Anti-human CD20 (Rituximab Biosimilar) Recombinant (purple) (clone QA20A02) activates CDC in a dose-dependent manner, similarly to another Rituximab Biosimilar from another supplier (green). This antibody has an IC50 range of 500-200 ng/mL.
  • C_QA20A02_ULEAF_CD20_Antibody_Glycan1_031723
    N-glycan profile from two different lots (Lot A and Lot B) of anti-human CD20 (rituximab biosimilar, RUO) recombinant, clone QA20A02. Chromatograms of glycosylation show lot-to-lot consistency. G0= N-glycan without galactosylation, G0F=Fucosylated without galactosylation, G1Fa and G1Fb= N-glycan with one terminal galactosylation and fucosylation, G2F= N-glycan fucosylated with both terminal galactosylation, Man5= N-linked mannose-5 glycan
  • D_QA20A02_ULEAF_CD20_Antibody_Glycan2_031723
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378003 100 µg £185
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378004 1 mg £509
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD20 is a surface marker expressed on B lymphocytes. CD20 is a 33-37 kD protein with four hydrophobic transmembrane domains that it associates with cell-surface and cytoplasmic proteins such as CD53, CD81, CD82, MHCII, CD40, CBP, and BCR. This association contributes to signal transduction essential for the development, differentiation, and activation of B cells. CD20 is also variably expressed by B cell malignancies such as Chronic lymphocytic leukemia (CLL), follicular lymphoma, and diffuse large B-cell lymphoma (DLBCL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Human
Immunogen
Human CD20
Formulation
150 mM sodium chloride, pH 6.5
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
CMCD - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For Complement-Dependent Cytotoxicity assays the IC50 range is 0.2 - 0.5 µg/mL. It is recommended that the reagent is titrated for optimal performance for each application.

Application Notes

This recombinant antibody is based on the published sequence for the Rituximab antibody. This product is for research use only.  It is not intended for therapeutic or diagnostic purposes.  

RRID
AB_2924569 (BioLegend Cat. No. 378003)
AB_2924569 (BioLegend Cat. No. 378004)

Antigen Details

Structure
CD20 protein has four membrane-spanning domains with both the amino and carboxy termini facing the cytosol
Distribution

B cell, T cell subset

Function
The function of CD20 involves the development, differentiation, and activation of B cells
Interaction
CD20 associates with CD53, CD81, CD82, MHCII, CD40, CBP, BCR, and Src family kinases
Ligand/Receptor
The ligand for CD20 is unknown
Molecular Family
CD Molecules
Antigen References
  1. Li H, et al. 2003. J Biol Chem. 278:42427-34.
  2. Pavlasova G, et al. 2020. Haematol. 105:1494-1506.
  3. Kuek E, et al. 2016. Immunol Cell Biol. 94:11-23.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 03/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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