Ultra-LEAF™ Purified anti-human CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
W21097B (See other available formats)
Regulatory Status
RUO
Other Names
CD226, Platelet and T-cell activation antigen 1, PTA1, T lineage specific activation antigen 1 antigen
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
W21097B_ULEAF_CD226_Antibody_070224
Biotinylated human CD155-Fc chimera (Cat. No. 796604) (black circles) binds to immobilized recombinant human DNAM-1/CD226-Fc chimera (Cat. No. 773606). Ultra-LEAF™ purified anti-human CD226 (DNAM-1) (clone W21097B) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. This antibody blocks the binding of 0.2 μg/mL biotinylated human CD155-Fc chimera to 1 µg/mL immobilized recombinant human DNAM-1/CD226-Fc chimera. ND50 range: 0.02 - 0.15 μg/mL.
  • W21097B_ULEAF_CD226_Antibody_070224
    Biotinylated human CD155-Fc chimera (Cat. No. 796604) (black circles) binds to immobilized recombinant human DNAM-1/CD226-Fc chimera (Cat. No. 773606). Ultra-LEAF™ purified anti-human CD226 (DNAM-1) (clone W21097B) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. This antibody blocks the binding of 0.2 μg/mL biotinylated human CD155-Fc chimera to 1 µg/mL immobilized recombinant human DNAM-1/CD226-Fc chimera. ND50 range: 0.02 - 0.15 μg/mL.
Cat # Size Price Quantity Check Availability Save
639753 100 µg £301
Check Availability


Need larger quantities of this item?
Request Bulk Quote
639754 1 mg £784
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

DNAM-1, also known as CD226, was first identified as T lineage-specific activation antigen (TLiSA) and subsequently renamed as Platelet and T-cell activation antigen 1 (PTA). DNAM-1 consists of two Ig-like domains on its extracellular portion, a transmembrane region and a cytoplasmic region containing binding motifs for members of band 4.1 family and membrane-associated guanylate kinase homolog (MAGUK) family. DNAM-1 is predominantly expressed on cell surface of T cells, NK cells, monocytes/ macrophages, platelets and megakaryocytes and a subset of B cells. DNAM-1 serves as a signaling-transducing adhesion molecule, and is physically and functionally associates with LFA-1 (CD11a/CD18) on NK and T cells. The clustering of LFA-1 induces Fyn-mediated phosphorylation of DNAM-1 and signaling. Evidence suggests that DNAM-1 is involved in NK and T cell-mediated cytotoxicity (CTL) against ligand-expressing tumor cells. Two ligands for DNAM-1 have been identified, the poliovirus receptor (PVR/CD155) and its family member nectin-2 (PRR/CD112), which are located at cell junctions and broadly expressed on epithelial, endothelial and neuronal cells. Additionally, DNAM-1 regulates monocyte extravasation through PVR expressed by endothelial cells. It has been reported that DNAM-1 is specifically expressed on differentiated Th1 cells, but not to Th2 or Th0 cells. Antibody blocking assay demonstrates that DNAM-1 may play a role in polarization of T cells, activation and effectors of Th1 cells. PVRIG (CD112R) is identified as a novel immune checkpoint to inhibit T cell response by competing DNAM-1 with CD112.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human DNAM-1/CD226-Fc Chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.2 µg/mL recombinant biotinylated human CD155-Fc chimera (Cat. No 796604) to immobilized 1 µg/mL recombinant human DNAM-1/CD226-Fc chimera (Cat. No. 773606). ND50 range: 0.02 - 0.15 µg/mL. It is recommended that the reagent be  titrated for optimal performance for each application.

RRID
AB_3662324 (BioLegend Cat. No. 639753)
AB_3662324 (BioLegend Cat. No. 639754)

Antigen Details

Structure
Disulfide bond-linked homodimer, Ig domain containing adhesion molecule
Distribution

Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells; plasma membrane

Function
It is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cells.
Interaction
Epithelial, endothelial and neuronal cells; LFA-1
Ligand/Receptor
PVR (CD155) and Nectin-2 (CD112)
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Fuchs A, Colonna M. 2006. Semin Cancer Biol. 16:359-66.
  2. Kojima H, et al. 2003. J Biol Chem. 278:36748-53.
  3. Burns GF, et al. 1985. J Exp Med. 161:1063-78.
  4. Scott JL, et al. 1989. J Biol Chem. 264:13475-82.
  5. Shibuya A, et al. 1996. Immunity. 4:573-81.
  6. Shibuya K et al. 1999. Immunity. 11:615-23.
  7. Ralston KJ, et al. 2004. J Biol Chem. 279:33816-28.
  8. Tahara-Hanaoka S, et al. 2006. Blood. 107:1491-6.
  9. Bottino C, et al. 2003. J Exp Med. 198:557.
  10. Reymond N, et al. 2004. J Exp Med. 199:1331-41.
  11. Dardalhon V, et al. 2005. J Immunol. 175:1558-65.
  12. Shibuya K, et al. 2003. J Exp Med. 198:1829-39.
Gene ID
10666 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/02/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account