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Ultra-LEAF™ Purified anti-human CD27 Antibody

Pricing & Availability
Clone
W22076F (See other available formats)
Regulatory Status
RUO
Other Names
CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22076F_ULEAF_CD27_Block_030824
Recombinant human CD27L (Cat. No. 553404) (black circles) binds to immobilized recombinant human CD27 (TNFRSF7)-Fc chimera (Cat. No. 766004). Ultra-LEAF™ purified anti-human CD27 (clone W22076F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.1 µg/mL human CD27L to 2.0 µg/mL immobilized recombinant human CD27 (TNFRSF7)-Fc chimera. ND50: 0.08 - 0.5 µg/mL.
  • W22076F_ULEAF_CD27_Block_030824
    Recombinant human CD27L (Cat. No. 553404) (black circles) binds to immobilized recombinant human CD27 (TNFRSF7)-Fc chimera (Cat. No. 766004). Ultra-LEAF™ purified anti-human CD27 (clone W22076F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.1 µg/mL human CD27L to 2.0 µg/mL immobilized recombinant human CD27 (TNFRSF7)-Fc chimera. ND50: 0.08 - 0.5 µg/mL.
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636553 100 µg £295
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636554 1 mg £777
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD27 (TNFRSF7) is a member of the TNFR family. It is a type I membrane glycoprotein with a MW of 55KDa that forms a disulfide linked homodimer. The receptor possesses three cysteine rich repeats in the extracellular domain, the transmembrane region, and the intracellular domain. CD27 binds CD27L (CD70), another member of the TNF ligand family that forms a trimer; thus, it has been speculated that the CD27L binds to a trimeric structure composed by three dimeric CD27 molecules. The interaction between CD27 and its ligand, CD70, plays a role in providing co-stimulation for prolonged lymphocyte survival, enhanced T-cell proliferation, and memory-cell formation. T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. Soluble CD27 (sCD27), the extracellular domain of membrane-bound CD27, can be released after lymphocyte activation by differential splicing of the receptor protein or shedding from the cell surface by metalloproteinases (MMPs). Soluble CD27 has been detected in serum, plasma, and urine samples from healthy individuals, and it has been described elevated sCD27 levels in serum of patients with systemic lupus erythematosus, viral infections, and lymphoid malignancies. The sCD27-pool in cancer patients can be significantly enlarged by immunotherapy. CD27 is recognized as a marker for memory B cells and is considered of diagnostic value in patients who have decreased numbers of switched memory B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD27 (TNFRSF7)-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.1 µg/mL recombinant human CD27L (Cat. No. 553404) to immobilized 2 µg/mL recombinant human CD27 (TNFRSF7)-Fc chimera (Cat. No. 766004). ND50 range: 0.08 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_3106082 (BioLegend Cat. No. 636553)
AB_3106082 (BioLegend Cat. No. 636554)

Antigen Details

Structure
Homodimer
Distribution

Expressed on resting T and B cells, NK cells, and hematopoietic progenitor cells

Function
Induces T-cell costimulation and B-cell activation, plasma cell differentiation
Interaction
Activated T and B cells, APC
Ligand/Receptor
CD27L (CD70)
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Camerini D, et al. 1991 J Immunol. 147:3165-9.
  2. Prasad KV, et al. 1997. Proc Natl Acad Sci. 94:6346.
  3. Kato K, et al. 2007. Exp Hematology. 35:434-42.
  4. van Montfrans J M, et al. 2012. J Allergy Clin Immun. 129: 787-793.
  5. Goto N, et al. 2012. Leuk Lymphoma. 53:1494-500.
  6. Huang J, et al. 2013. J Immunol. 190:6250-8.
Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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