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Ultra-LEAF™ Purified anti-human CD28 Recombinant Antibody

Pricing & Availability
Clone
QA17A12 (See other available formats)
Regulatory Status
RUO
Workshop
V-CD28.05
Other Names
T44, Tp44
Isotype
Human IgG1, κ
Ave. Rating
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Product Citations
publications
A_QA17A12_ULEAF_CD28_1_010824
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone SK7) APC and Ultra-LEAF™ purified anti-human CD28 recombinant (clone QA17A12) (left) or Ultra-LEAF™ purified human IgG1, κ isotype control (right), followed by biotinylated anti-human IgG and streptavidin PE.
  • A_QA17A12_ULEAF_CD28_1_010824
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone SK7) APC and Ultra-LEAF™ purified anti-human CD28 recombinant (clone QA17A12) (left) or Ultra-LEAF™ purified human IgG1, κ isotype control (right), followed by biotinylated anti-human IgG and streptavidin PE.
  • B_QA17A12_ULEAF_CD28_2_010824
    Human peripheral blood T cells isolated from PBMCs using the MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480021) were labeled with CFSE (Cat. No. 423801), then stimulated with plate-bound Ultra-LEAF™ purified anti-human CD3 (clone UCHT1) and Ultra-LEAF™ purified anti-human CD28 recombinant (clone QA17A12) (filled histogram), Ultra-LEAF™ purified anti-human CD28 (clone CD28.2) (open dotted histogram), or Ultra-LEAF™ purified human IgG1 isotype control (open dashed histogram) for 4 days. Data shown are gated on CD4+ cells.
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377203 100 µg £76
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377204 1 mg £102
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus, Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type
Recombinant
Host Species
Human
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Costim - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA17A12 completely blocks binding of parental clone.

Clone QA17A12 activates human T cells in co-stimulation assay with CD3 as efficiently as parental clone.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Nunes J, et al. 1993. Biochem J. 293:835-42.
  3. Galea-Lauri J, et al. 1999. J Immunol. 163:62-70.
  4. Tazi A, et al. 1999. J Immunol. 163:3511-5. (IHC)
  5. Marti F, et al. 2001. J Immunol. 166:197-206. (Costim)
  6. Jeong SH, et al. 2004. J Virol. 78:6995-7003. (Costim)
  7. Rivollier A, et al. 2004. Blood. 104:4029-37. (Costim)
  8. Scharschmidt E, et al. 2004. Mol Cell Biol. 24:3860-73. (Costim)
  9. Sheng W, et al. 2006. FEBS Lett. 580:6819-24. PubMed
  10. Mitsuhashi M. 2007. Clin Chem. 53:148-9. PubMed
  11. Ye Z, et al. 2008. Infect Immun. 76:2541-50. PubMed
  12. Magatti M, et al. 2008. Stem Cells. 26:182-92. (FA) PubMed
  13. Yoshino N, et al. 2000. Exp Anim. 49:97-110. (FC)
  14. Berg M, et al. 2008. J Leukoc Biol. 83:853-63. (IP) PubMed
  15. Rout N, et al. 2010. PLoS One. 5:e9787. (FC)
  16. Leonard JA, et al. 2011. J Virol. 85:6867-81. PubMed
  17. Nomura T, et al. 2012. J Virol. 86:6481-90. PubMed
RRID
AB_3097476 (BioLegend Cat. No. 377203)
AB_3097476 (BioLegend Cat. No. 377204)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

Function
T cell costimulation
Interaction
B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Ligand/Receptor
CD80, CD86
Cell Type
T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linskey PS, et al. 1993. Annu Rev Immunol. 11:191-212.
Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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