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Ultra-LEAF™ Purified anti-human CD28 Recombinant Antibody

Pricing & Availability
Clone
S20013F.RecH4 (See other available formats)
Regulatory Status
RUO
Other Names
T44, Tp44
Isotype
Human IgG4, κ
Ave. Rating
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Product Citations
publications
S20013F-RecH4_ULEAF_CD28_Antibody_1_091724
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and Ultra-LEAF™ purified anti-human CD28 recombinant (clone 20013F.RecH4) (left) or Ultra-LEAF™ purified human IgG4, κ isotype control (right) followed by anti-human IgG Fc APC.
  • S20013F-RecH4_ULEAF_CD28_Antibody_1_091724
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and Ultra-LEAF™ purified anti-human CD28 recombinant (clone 20013F.RecH4) (left) or Ultra-LEAF™ purified human IgG4, κ isotype control (right) followed by anti-human IgG Fc APC.
  • S20013F-RecH4_ULEAF_CD28_Antibody_2_091724
    Human peripheral blood T cells were isolated using MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480022), and were labeled using the CFSE Cell Division Tracker Kit (Cat. No. 423801). Cells were stimulated with sub-optimal, plate-bound Ultra-LEAF™ purified anti-human CD3 (clone UCHT1) and Ultra-LEAF™ purified anti-human CD28 recombinant (clone S20013F.RecH4) (filled histogram) or Ultra-LEAF™ purified human IgG4, κ isotype control (open histogram) for 4 days.
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350902 100 µg £295
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Human
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Activ - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S20013F.RecH4 is able to completely block binding of clone S20013F.

Clone S20013F.RecH4 can co-stimulate anti-CD3 induced human peripheral T-cell proliferation.

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV positive B cells

Function
T cell costimulation
Ligand/Receptor
CD80/CD86
Cell Type
T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman SF, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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