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Ultra-LEAF™ Purified anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
5.1H11 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5.1H11_ULEAF_CD56_Antibody_FC_080815
Human peripheral blood lymphocytes were stained with CD56 (clone 5.1H11) Ultra-LEAF™ purified (filled histogram) or mouse IgG1, κ Ultra-LEAF™ purified isotype control (open histogram), followed by anti-mouse IgG PE.
  • 5.1H11_ULEAF_CD56_Antibody_FC_080815
    Human peripheral blood lymphocytes were stained with CD56 (clone 5.1H11) Ultra-LEAF™ purified (filled histogram) or mouse IgG1, κ Ultra-LEAF™ purified isotype control (open histogram), followed by anti-mouse IgG PE.
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362548 500 µg £298
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myotube cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Walsh FS, et al. 1981. Nature 289:60. (FC)
  2. Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC)
  3. Pavlath GK, et al. 1989. Nature 337:570. (FC)
  4. Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations
  1. Li H, et al. 2020. Mol Ther Methods Clin Dev. 1.436111111. PubMed
  2. Cerignoli F, et al. 2018. PLoS One. 13:e0193498. PubMed
  3. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  4. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  5. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
RRID
AB_2565995 (BioLegend Cat. No. 362548)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD56 (NCAM) Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
PerCP/Cyanine5.5 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
APC/Cyanine7 anti-human CD56 (NCAM) 5.1H11 FC
PE/Cyanine5 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 647 anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 488 anti-human CD56 (NCAM) 5.1H11 FC
Pacific Blue™ anti-human CD56 (NCAM) 5.1H11 FC
Alexa Fluor® 700 anti-human CD56 (NCAM) 5.1H11 FC
PerCP anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 650™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 510™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 421™ anti-human CD56 (NCAM) 5.1H11 FC
Biotin anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 605™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 570™ anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 711™ anti-human CD56 (NCAM) 5.1H11 FC
PE/Dazzle™ 594 anti-human CD56 (NCAM) 5.1H11 FC
FITC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 785™ anti-human CD56 (NCAM) 5.1H11 FC
Ultra-LEAF™ Purified anti-human CD56 (NCAM) 5.1H11 FC
APC/Fire™ 750 anti-human CD56 (NCAM) 5.1H11 FC
PE anti-human CD56 (NCAM) 5.1H11 FC
APC anti-human CD56 (NCAM) 5.1H11 FC
Brilliant Violet 750™ anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-A0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-C0047 anti-human CD56 (NCAM) 5.1H11 PG
TotalSeq™-B0047 anti-human CD56 (NCAM) 5.1H11 PG
Spark NIR™ 685 anti-human CD56 (NCAM) 5.1H11 FC
KIRAVIA Blue 520™ anti-human CD56 (NCAM) 5.1H11 FC
GMP PE anti-human CD56 (NCAM) 5.1H11 FC
TotalSeq™-D0047 anti-human CD56 (NCAM) 5.1H11 PG
GMP APC anti-human CD56 (NCAM) 5.1H11 FC
Spark YG™ 593 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD56 (NCAM) SF 5.1H11 FC
Spark Red™ 718 anti-human CD56 (NCAM) 5.1H11 FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF 5.1H11 FC
Go To Top Version: 1    Revision Date: 08/19/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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