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Ultra-LEAF™ Purified anti-human CXCL2 (Groβ) Antibody

Pricing & Availability
Clone
A16108E (See other available formats)
Regulatory Status
RUO
Other Names
Gro2, Macrophage inflammatory protein (MIP-2)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16108E_ULEAF_CXCL2_Grobeta_Antibody_071221
Recombinant human CXCL2 (Groβ) (Cat. No. 582006) (black circles), induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL2 (Groβ) antibody (clone A16108E) (purple squares) neutralizes the chemotaxis of recombinant human CXCL2 (Groβ) on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.2 - 1 µg/mL.
  • A16108E_ULEAF_CXCL2_Grobeta_Antibody_071221
    Recombinant human CXCL2 (Groβ) (Cat. No. 582006) (black circles), induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL2 (Groβ) antibody (clone A16108E) (purple squares) neutralizes the chemotaxis of recombinant human CXCL2 (Groβ) on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.2 - 1 µg/mL.
  • A16108E_ULEAF_CXCL2_Grobeta_Antibody_2_121923
    Direct ELISA showing purified anti-human CXCL2 antibody (clone A16108E) bound to plate-immobilized recombinant human CXCL2 (Groβ) protein (Cat. No. 582002). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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311001 100 µg £185
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311002 1 mg £509
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CXCL2 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor; this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8). CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and it has been detected in serum of patients with this disease. Also, CXCL1, CXCL2 and CXCL3 have been shown to be highly expressed in patients with malignant melanoma. In addition, CXCL2 and CXCL1 are upregulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, it has been shown that transfer of encephalitogenic CD4+ Th17 cells induces CXCL1 and CXCL2 transcription in the spinal cords of naïve, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in MS and EAE. In fact, it has been shown that CXCL1 and CXCL2 are upregulated in the preclinical and clinical stages of EAE.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CXCL2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL2 (Groβ) on mouse Baf3-mCXCR2 transfectant chemoattractant cells. ND50 range: 0.2 - 1 µg /mL. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (A16108E) could function as capture antibody when paired with Purified anti-human CXCL2 antibody (clone Poly5211) as detection antibody using Recombinant Human CXCL2 (Groβ) protein (Cat. No. 582002) as assay standard.

RRID
AB_2894458 (BioLegend Cat. No. 311001)
AB_2894458 (BioLegend Cat. No. 311002)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, monocytes, fibroblasts, and epithelial cells.

Function
CXCL2 chemoattracts and activates neutrophils, and it plays a key role in neutrophil mobilization from the bone marrow. CXCL2 is induced by TNFα, IL-1α, IL-17, and LPS.
Interaction
Neutrophils, basophils, lymphocytes, monocytes, keratinocytes, hematopoitic cells, endothelial cells, bone marrow endolthelial cells, osteoblasts, epithelial cells, fibroblasts, melanoma cells, melanocytes, and neurons
Ligand/Receptor
CXCR2
Cell Type
Epithelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Haskill S, et al. 1990. P. Natl. Acad. Sci. USA 87:7732.
  2. De Plaen I, et al. 2006. Immunology 118: 153.
  3. Carlson T, et al. 2008. J. Exp. Med. 205:811.
  4. Eash KJ, et al. 2010. J. Clin. Invest. 120:2423.
  5. Keeley EC, et al. 2011. Exp. Cell. Res. 317: 685.
  6. Onishi RM and Gaffen SL, 2011. Immunology 129:311.
Gene ID
2920 View all products for this Gene ID
UniProt
View information about CXCL2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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