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Ultra-LEAF™ Purified anti-human EGFR Antibody

Pricing & Availability
Clone
W21030C (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21030C_ULEAF_EGFR_Antibody_1_013124
Recombinant human EGF (Cat. No. 585506) (black circles) inhibits proliferation in human epithelial A431 cells. Ultra-LEAF™ purified anti-human EGFR (clone W21030C) (purple squares) blocks the inhibitory effect of recombinant human EGF, at 10 ng/mL, in the proliferation of human epithelial A431 cells in a dose-dependent manner; whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have effect. ND50 range: 0.15 - 1.5 µg/mL.
  • W21030C_ULEAF_EGFR_Antibody_1_013124
    Recombinant human EGF (Cat. No. 585506) (black circles) inhibits proliferation in human epithelial A431 cells. Ultra-LEAF™ purified anti-human EGFR (clone W21030C) (purple squares) blocks the inhibitory effect of recombinant human EGF, at 10 ng/mL, in the proliferation of human epithelial A431 cells in a dose-dependent manner; whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have effect. ND50 range: 0.15 - 1.5 µg/mL.
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630953 100 µg £373
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630954 1 mg £897
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human EGFR
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the inhibitory effect of recombinant human EGF (Cat. No. 585506) at 10 ng/mL in human epithelial A431 cell proliferation. ND50 range: 0.15 - 1.5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3106023 (BioLegend Cat. No. 630953)
AB_3106023 (BioLegend Cat. No. 630954)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Ligand/Receptor
EGF, TGFA/TGF-alpha, AREG, Epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Hunter T & Cooper JA. 1981. Cell. 24:741-52.
  2. Gill GN & Lazar CS. 1981. Nature. 293:305-7.
  3. Reynolds Jr FH, et al. 1981. Nature. 292:259-62.
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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