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Ultra-LEAF™ Purified anti-human G-CSF Antibody

Pricing & Availability
Clone
W20129F (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte-Colony Stimulating Factor, CSF3, CSF-β, Pluripoietin, Lenograstim, Filgrastim
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20129F_ULEAF_G_CSF_Antibody_071723
Recombinant human G-CSF (Cat. No. 578602) (black circles) induces proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-human G-CSF (clone W20129F) (purple squares) neutralizes M-NFS-60 cell proliferation induced by recombinant human G-CSF at 0.2 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range is 0.08 - 1.0 µg/mL.
  • W20129F_ULEAF_G_CSF_Antibody_071723
    Recombinant human G-CSF (Cat. No. 578602) (black circles) induces proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-human G-CSF (clone W20129F) (purple squares) neutralizes M-NFS-60 cell proliferation induced by recombinant human G-CSF at 0.2 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range is 0.08 - 1.0 µg/mL.
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619953 100 µg £295
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619954 1 mg £825
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells, it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human G-CSF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing M-NFS-60 cell proliferation induced by recombinant human G-CSF (Cat. No. 578602) at 0.2 ng/mL. ND50 range: 0.08 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068200 (BioLegend Cat. No. 619953)
AB_3068200 (BioLegend Cat. No. 619954)

Antigen Details

Structure
Heterodimer
Distribution

Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells

Function
G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxide anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction
Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell lines
Ligand/Receptor
CD114 (G-CSFR)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Nagata S, et al. 1986. Nature. 319:415-8.
  2. Kim HK, et al. 2006. Blood. 108:812-20.
  3. Donahue RE, et al. 2009. Blood. 114:2530-41.
  4. Shimoji K, et al. 2010. Cell Stem Cell. 6:227-37.
  5. Martins A, et al. 2010. IUBMB Life. 62:611-7.
  6. Hara M, et al. 2011. J Exp Med. 208:715-27.
Gene ID
1440 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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