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Ultra-LEAF™ Purified anti-human IFN-α2 Antibody

Pricing & Availability
Clone
W20057A (See other available formats)
Regulatory Status
RUO
Other Names
IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20057A_ULEAF_IFN-a2_Antibody_1_061924
Recombinant human IFN-α2 (Cat. No. 592702) (black circles) inhibits the production of human MCP-1/CCL2 (Cat. No. 438804) in U937 cells. Ultra-LEAF™ purified anti-human IFN-α2 (clone W20057A) (purple squares) neutralizes the inhibition effect of recombinant human IFN-α2 (Cat. No. 592702) at 1 ng/mL on production of human MCP-1/CCL2 induced in U937 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified Rat IgG2a, κ isotype Control (gray triangles) (Cat. No. 400544) does not have effect. ND50 range: 4.5 - 30 ng/mL.
  • W20057A_ULEAF_IFN-a2_Antibody_1_061924
    Recombinant human IFN-α2 (Cat. No. 592702) (black circles) inhibits the production of human MCP-1/CCL2 (Cat. No. 438804) in U937 cells. Ultra-LEAF™ purified anti-human IFN-α2 (clone W20057A) (purple squares) neutralizes the inhibition effect of recombinant human IFN-α2 (Cat. No. 592702) at 1 ng/mL on production of human MCP-1/CCL2 induced in U937 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified Rat IgG2a, κ isotype Control (gray triangles) (Cat. No. 400544) does not have effect. ND50 range: 4.5 - 30 ng/mL.
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638253 100 µg £295
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638254 1 mg £777
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Description

Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells. In a preliminary study, IFN-α2 appears to demonstrate effective immunostimulatory properties impacting clinical outcomes in tongue squamous cell carcinoma patients. IFN-α has also been used in the treatment of chronic hepatitis C (CHC) despite its relatively instability and need for frequent parenteral administration. Although pegylation reduces IFN-α in vitro activity, polyethylene glycol (PEG)-IFN-α increases the stability and plasma half-life of IFN-α resulting in PEG-IFN-α replacing native IFN-α in CHC treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human IFN-α2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the production of human MCP-1/CCL2 induced by recombinant human IFN-α2 at 1 ng/mL in U937 cells. ND50 range: 4.5 - 30 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3662319 (BioLegend Cat. No. 638253)
AB_3662319 (BioLegend Cat. No. 638254)

Antigen Details

Distribution

IFN-α is ubiquitously expressed.

Function
IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
Interaction
Varieties of cells express the IFNα receptor (IFNAR).
Ligand/Receptor
IFNAR complex has two components, IFNAR1 and IFNAR2.
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Bose A & Baral R. 2007. Immunol Lett. 108:68-77.
  2. Zhao W, et al. 2008. J Immunol. 180:5483-9.
  3. Badiger R, et al. 2012. PLoS One. 7:e46779.
  4. Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485-94.
  5. Becker-Merok A, et al. 2013. Lupus. 22:155-63.
  6. Mukherjee KK, et al. 2012. Indian J Med Res. 136:54-9.
Gene ID
3440 View all products for this Gene ID
UniProt
View information about IFN-alpha2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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