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Ultra-LEAF™ Purified anti-human IL-16 Antibody

Pricing & Availability
Clone
14.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-16
Isotype
Mouse IgG2a
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Product Citations
publications
14.1_UltraLeaf_IL-16_Antibody_FC_041714
Human peripheral blood lymphocytes were fixed, permeabilized, and stained with Ultra-LEAF™ purified anti-human IL-16 (clone 14.1, filled histogram) or purified mouse IgG2a, κ (open histogram), followed by anti-mouse IgG PE.
  • 14.1_UltraLeaf_IL-16_Antibody_FC_041714
    Human peripheral blood lymphocytes were fixed, permeabilized, and stained with Ultra-LEAF™ purified anti-human IL-16 (clone 14.1, filled histogram) or purified mouse IgG2a, κ (open histogram), followed by anti-mouse IgG PE.
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519108 1 mg £524
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Description

Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
  2. Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
  3. Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
  4. Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
  5. Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
RRID
AB_2563454 (BioLegend Cat. No. 519108)

Antigen Details

Structure
12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution

Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.

Function
Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor
CD4.
Cell Type
Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.

Gene ID
3603 View all products for this Gene ID
UniProt
View information about IL-16 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 04/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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