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Ultra-LEAF™ Purified anti-human IL-22 Antibody

Pricing & Availability
Clone
A16057A (See other available formats)
Regulatory Status
RUO
Other Names
TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A16057A_ULEAF_IL-22_Antibody_1_082219
Recombinant human IL-22 (Black circles, Cat. No. 571306) stimulates the production of IL-10 in human Colo 205 cells. Anti-human IL-22 antibody (clone A16057A, purple squares) inhibits the effect of recombinant human IL-22 at 0.75 ng/mL in human Colo 205 cells in a dose-dependent manner. The ND50 = 1.2 - 6 μg/mL.
  • A16057A_ULEAF_IL-22_Antibody_1_082219
    Recombinant human IL-22 (Black circles, Cat. No. 571306) stimulates the production of IL-10 in human Colo 205 cells. Anti-human IL-22 antibody (clone A16057A, purple squares) inhibits the effect of recombinant human IL-22 at 0.75 ng/mL in human Colo 205 cells in a dose-dependent manner. The ND50 = 1.2 - 6 μg/mL.
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618303 100 µg £185
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618304 1 mg £490
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human IL-22
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the neutralization of IL-10 production induced by human recombinant IL-22 (0.75 ng/mL) on human Colo 205 cells. ND50 range: 1.2 - 6.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814466 (BioLegend Cat. No. 618303)
AB_2814466 (BioLegend Cat. No. 618304)

Antigen Details

Structure
Cytokine
Distribution

Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells

Function
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains
Cell Type
Epithelial cells, NK cells, T cells, Th17
Biology Area
Cell Biology, Immunology, Neuroinflammation, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Nagalakshmi ML, et al. 2004. Intern Immunopharmacol. 4:679-691.
  2. Kebir H, et al. 2007. Nat Med. 13:1173-1175.
  3. Gu Y, et al. 2008. Eur J Immunol. 38:1807-1813.
  4. Pene J, et al. 2008. J Immunol. 180:7423-7430.
  5. Dumotier L, et al. 2000. J immune 164:1814-1819.
  6. Xie MH, et al. 2000. J Biol Chem. 765:31335-31339.
  7. Jones BC, et al. 2008. Structure. 16:1333-44
  8. Kotenko SV, et al. 2001. J Immunol. 166:7096-71033.
  9. Chang C, et al. 2006. Cell Res 16:902-907.
Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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