Ultra-LEAF™ Purified anti-human IL-3 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant human IL-3

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

Block - Quality tested
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED₅₀ = 0.02 – 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA^1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting⁴ and immunoprecipitation⁴.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
2. Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
3. Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
4. Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
5. Mahanty S, et al. 1992. J. Immunol. 148:3567.

RRID

AB_2616770 (BioLegend Cat. No. 500506)

Antigen Details

Structure

Cytokine; 14-30 kD (Mammalian)

Bioactivity

Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.

Cell Sources

Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.

Cell Targets

Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.

Receptors

IL-3R, α subunit (CD123), common β subunit (CDw131).

Biology Area

Cell Biology, Stem Cells

Molecular Family

Growth Factors, Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.

Regulation

Upregulated by T cell stimulation; downregulated by glucocorticoids.

Gene ID

3562 View all products for this Gene ID

UniProt

View information about IL-3 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis