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Ultra-LEAF™ Purified anti-human TNFSF18 (GITRL) Antibody

Pricing & Availability
Clone
W21092F (See other available formats)
Regulatory Status
RUO
Other Names
TL6, AITRL, GITRL, GITR Ligand, Tumor necrosis factor superfamily, Member 18
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W21092F_ULEAF_TNFSF18_Block_052224
Recombinant Human TNFSF18 (GITRL) chimera (Cat. No. 559204) (black circles) binds to immobilized human GITR (TNFRSF18) Fc chimera (Cat. No. 764302). Ultra-LEAF™ purified anti-human TNFSF18 (GITRL) (clone W21092F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.15 µg/mL recombinant human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc chimera. ND50 range: 0.12 - 1 µg/mL.
  • W21092F_ULEAF_TNFSF18_Block_052224
    Recombinant Human TNFSF18 (GITRL) chimera (Cat. No. 559204) (black circles) binds to immobilized human GITR (TNFRSF18) Fc chimera (Cat. No. 764302). Ultra-LEAF™ purified anti-human TNFSF18 (GITRL) (clone W21092F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.15 µg/mL recombinant human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc chimera. ND50 range: 0.12 - 1 µg/mL.
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637853 100 µg £295
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637854 1 mg £777
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Description

TNFSF18 is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC) including macrophages, B cells, dendritic cells (DCs), and endothelial cells. Its expression increases upon the stimulation of proinflammatory stimuli. The receptor of TNFSF18 is the glucocorticoid-induced TNFR-related protein (GITR), which is a member of the TNFR superfamily. Engagement of TNFSF18 and GITR leads to T cell activation, proliferation, and cytokine production. It also inhibits the suppressive function of T regulatory cells and induces macrophage activation. GITR-TNFSF18 interaction plays an essential role in the regulation of the immune response to self-antigens and pathogens. In addition, this receptor-ligand interaction has been shown to modulate anti-tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human TNFSF18 (GITRL)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.15 µg/mL human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc chimera. ND50 range: 0.12 – 1 mg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3106203 (BioLegend Cat. No. 637853)
AB_3106203 (BioLegend Cat. No. 637854)

Antigen Details

Structure
Monomer
Distribution

Macrophages, B cells, dendritic cells (DCs), and endothelial cells

Function
TNFSF18 plays roles in regulating T cell activation and natural killer cell anti-tumor activity. It is induced by inflammatory processes.
Interaction
T cells
Ligand/Receptor
GITR (TNFRSF18)
Cell Type
Embryonic Stem Cells
Biology Area
Cell Proliferation and Viability, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Yu KY, et al. 2003. Biochem Biophys Res Commun. 310:433-8.
  2. Kim JD, et al. 2003. Genes Immun. 4:564-9.
  3. Azuma M. 2010. Crit Rev Immunol. 30:547-57.
  4. Hanabuchi S, et al. 2006. Blood. 107:3617-23.
  5. Muriglan SJ, et al. 2004. J Exp Med. 200:149-57.
  6. Shevach EM and Stephens GL. 2006. Nat Rev Immunol. 6:613-8.
  7. Baltz K, et al. 2007. FASEB J. 21:2442-54.
  8. Placke T, et al. 2010. Clin Dev Immunol. 2010:239083.
Gene ID
8995 View all products for this Gene ID
UniProt
View information about TNFSF18 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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